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The effect of a histone deacetylase inhibitor - valproic Acid - on nucleoli in human leukaemic myeloblasts.
MedLine Citation:
PMID:  21138651     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The present study was undertaken to provide more information on nucleolar changes induced by a histone deacetylase inhibitor such as valproic acid in leukaemic myeloblasts at the single-cell level. For this study, RNA in nucleoli was visualized by a simple but sensitive cytochemical procedure in unfixed cytospins of short-term bone marrow cultures from patients suffering from acute myeloid leukaemia. Valproic acid in leukaemic myeloblasts markedly reduced the nucleolar size and also produced significant transformation of "active" to "resting" and "inactive" nucleoli that reflected the alteration of the nucleolar transcription in sensitive myeloblasts. On this occasion it should be added that valproic acid significantly increased the incidence of altered myeloblasts that changed to apoptotic cells or apoptotic bodies and cell ghosts. In contrast to the above-mentioned decreased nucleolar size, the nucleolar RNA concentration, expressed by computerassisted RNA image densitometry in valproic acidtreated myeloblasts, was not significantly changed. The results of the present study clearly indicated that the nucleolar size and transformation of "active" to "sleeping" or "inactive" nucleoli are convenient markers of the sensitivity and alteration of leukaemic myeloblasts produced by a histone deacetylase inhibitor, valproic acid, at the single-cell level.
Authors:
K Smetana; M Zápotocký
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Folia biologica     Volume:  56     ISSN:  0015-5500     ISO Abbreviation:  Folia Biol. (Praha)     Publication Date:  2010  
Date Detail:
Created Date:  2010-12-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0234640     Medline TA:  Folia Biol (Praha)     Country:  Czech Republic    
Other Details:
Languages:  eng     Pagination:  201-5     Citation Subset:  IM    
Affiliation:
Institute of Haematology and Blood Transfusion, Prague, Czech Republic.
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