Document Detail

The effect of glycosaminoglycan stabilization on tissue buckling in bioprosthetic heart valves.
MedLine Citation:
PMID:  18199477     Owner:  NLM     Status:  MEDLINE    
Bioprosthetic valves are used in thousands of heart valve replacement surgeries. Existing glutaraldehyde-crosslinked bioprosthetic valves fail due to either calcification or degeneration. Glutaraldehyde crosslinking does not stabilize valvular glycosaminoglycans (GAGs). GAGs, predominantly present in the medial spongiosa layer of native heart valve cusps, play an important role in regulating physico-mechanical behavior of the native cuspal tissue during dynamic motion. The primary objective of this study was to identify the role of cuspal GAGs in valve tissue buckling. Glutaraldehyde-crosslinked cusps showed extensive buckling compared to fresh, native cusps. Removal of GAGs by treatment with GAG-degrading enzymes led to a marked increase in buckling behavior in glutaraldehyde-crosslinked cusps. We demonstrate that the retention of valvular GAGs by carbodiimide crosslinking together with chemical attachment of neomycin trisulfate (a hyaluronidase inhibitor), prior to glutaraldehyde crosslinking, reduces the extent of buckling in bioprosthetic heart valves. Furthermore, following exposure to GAG-digestive enzymes, neomycin-trisulfate-bound cusps experienced no alterations in buckling behavior. Such moderate buckling patterns mimicked that of fresh, untreated cusps subjected to similar bending curvatures. Thus, GAG stabilization may subsequently improve the durability of these bioprostheses.
Sagar R Shah; Naren R Vyavahare
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2008-01-15
Journal Detail:
Title:  Biomaterials     Volume:  29     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-02-11     Completed Date:  2008-04-24     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  1645-53     Citation Subset:  IM    
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MeSH Terms
Enzymes / metabolism
Glycosaminoglycans / chemistry*,  metabolism
Heart Valve Prosthesis*
Microscopy, Electron, Scanning
Grant Support
HL70969/HL/NHLBI NIH HHS; R01 HL070969/HL/NHLBI NIH HHS; R01 HL070969-01A2/HL/NHLBI NIH HHS; R01 HL070969-02/HL/NHLBI NIH HHS; R01 HL070969-03/HL/NHLBI NIH HHS; R01 HL070969-04/HL/NHLBI NIH HHS; R01 HL070969-05A1/HL/NHLBI NIH HHS
Reg. No./Substance:
0/Enzymes; 0/Glycosaminoglycans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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