Document Detail


The effect of gestational age and labour on markers of lipid and protein oxidation in cord plasma.
MedLine Citation:
PMID:  15104212     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
There are many potential sources of reactive oxidants around the time of birth and pre-term infants are considered to be particularly vulnerable to oxidative injury. To gain insight into these processes, we have measured biomarkers of lipid and protein oxidation in umbilical cord plasma and related concentrations to mode of delivery and gestational age. Protein carbonyls were measured by ELISA and malondialdehyde (MDA) by HPLC after reaction with thiobarbituric acid, for 54 pre-term (< or = 36 weeks gestational age) and 43 term infants. Protein carbonyls were significantly lower in pre-term (median for < 32 weeks gestational age 0.048nmol/mg protein) than in term infants (0.105 nmol/mg, p = 0.004), and were unrelated to mode of delivery. In contrast, MDA concentrations were higher in the very pre-term (<32 weeks gestation) group (2.47 compared with 1.83 microM for term infants, p < 0.0001). MDA concentrations were higher in infants who were born with labour compared with elective caesarean section. Pre-eclampsia in the mother was associated with higher cord blood MDA concentrations. The MDA results are consistent with other studies of this marker and could be interpreted as indicating increased oxidative stress associated with prematurity and labour. However, the lower protein carbonyls in pre-term infants would lead to an opposite interpretation. More information is needed on the source and fate of these and other biomarkers before drawing strong conclusions on how they reflect oxidative stress in this and other clinical situations.
Authors:
Tessa J Mocatta; Christine C Winterbourn; Terrie E Inder; Brian A Darlow
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Free radical research     Volume:  38     ISSN:  1071-5762     ISO Abbreviation:  Free Radic. Res.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-04-23     Completed Date:  2004-09-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9423872     Medline TA:  Free Radic Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  185-91     Citation Subset:  IM    
Affiliation:
Department of Pathology, Christchurch School of Medicine and Health Sciences, P.O. Box 4345, Christchurch, New Zealand.
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MeSH Terms
Descriptor/Qualifier:
Biological Markers / blood
Birth Weight
Blood Proteins / metabolism*
Chromatography, High Pressure Liquid
Enzyme-Linked Immunosorbent Assay
Female
Fetal Blood / metabolism*
Gestational Age*
Humans
Infant, Newborn
Infant, Premature / blood*,  metabolism
Labor, Obstetric*
Lipid Peroxidation*
Malondialdehyde / metabolism
Oxidation-Reduction
Oxidative Stress
Pregnancy
Pregnancy Complications / blood
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Blood Proteins; 542-78-9/Malondialdehyde

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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