| The effect of gastrin on growth of human stomach cancer cells. | |
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MedLine Citation:
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PMID: 1616389 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Gastrin is known as a trophic factor for some stomach and colorectal cancer cells; however, the roles of gastrin receptors and the intracellular signal transduction pathways by which gastrin regulates cell growth are still unknown. The authors examined the effect of synthetic human gastrin-17 on growth of human stomach cancer cells (the parent line, AGS-P, and two different clones, AGS-10 and AGS-12), which were established (and have been maintained) in our laboratory. Gastrin stimulated growth of AGS-P and AGS-10 cells, which have gastrin receptors, in a dose-dependent fashion. A highly selective gastrin receptor antagonist, JMV 320, inhibited the growth-stimulatory effect of gastrin on AGS-P cells in a dose-dependent fashion. Concentrations of gastrin (10(-8) to 10(-6) M), which stimulated growth of AGS-P cells, did not affect either cyclic adenosine monophosphate production or phosphatidylinositol hydrolysis. Gastrin (10(-11) to 10(-5) M) mobilized calcium from the intracellular organelles to increases intracellular calcium level in AGS-P cells. The AGS-12 clone has no gastrin receptors, and gastrin did not affect growth or mobilization of intracellular calcium in these cells. Our findings indicate that gastrin stimulates growth of AGS cells through a mechanism that involves binding to specific gastrin receptors that are linked to the system for mobilization of intracellular calcium. |
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Authors:
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J Ishizuka; J Martinez; C M Townsend; J C Thompson |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Annals of surgery Volume: 215 ISSN: 0003-4932 ISO Abbreviation: Ann. Surg. Publication Date: 1992 May |
Date Detail:
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Created Date: 1992-07-30 Completed Date: 1992-07-30 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0372354 Medline TA: Ann Surg Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 528-34 Citation Subset: AIM; IM |
Affiliation:
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Department of Surgery, University of Texas Medical Branch, Galveston 77550. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Calcium / metabolism Gastrins / pharmacology, physiology* Hormones / pharmacology Humans Molecular Sequence Data Receptors, Cholecystokinin / antagonists & inhibitors, drug effects Sincalide / analogs & derivatives, pharmacology Stomach Neoplasms / pathology* Tumor Cells, Cultured / drug effects* |
| Grant Support | |
ID/Acronym/Agency:
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5R37 DK 15241/DK/NIDDK NIH HHS; P01 DK 35608/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Gastrins; 0/Hormones; 0/Receptors, Cholecystokinin; 130582-10-4/JMV 320; 25126-32-3/Sincalide; 60748-06-3/gastrin 17; 7440-70-2/Calcium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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