| The effect of food chemicals on cell aging of human diploid cells in in vitro culture. | |
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MedLine Citation:
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PMID: 8246307 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The potency of food chemicals to induce cell aging was evaluated in human diploid fibroblast cells HAIN-55 having a finite replicative potential by using in vitro aging markers, i.e., decreases of maximum proliferative potential (lifespan) of cells, saturation density in monolayer culture (SD), plating efficiency (PE) and mitotic index (MI), and an increase of cells with polyploid karyotypes. By treatment twice with low concentration of genotoxic chemicals aflatoxin B1, allylisothiocyanate or trans-cinnamaldehyde (severe clastogenic flavoring agent; Kasamaki et al., 1982), lifespan (expressed by the number of cumulative cell population doubling (CPD)) of the treated cells was reduced by 8-12 CPDs accompanied by change of the other aging markers. By successive treatment (29 or 25 times) with non-genotoxic chemical aspartame (N-L-aspartyl-L-phenylalanine) or L-canavanine (structural analogue of L-arginine), lifespan of the treated cells was also slightly shortened (by 2-6 CPDs) compared with the untreated control cells. In the process of cell aging, Mitochondrial activity (MTT activity) decreased almost in parallel with the decrease of SD and MI. On the basis of these results, a variety of genotoxic and non-genotoxic chemicals were examined by using MTT activity as the aging marker for their effects on the aging of HAIN-55 cells and bovine artery endothelial cells which also had a finite replicative potential. The results showed that seven genotoxic and nine non-genotoxic chemicals promoted cell aging. |
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Authors:
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A Kasamaki; S Urasawa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of toxicological sciences Volume: 18 ISSN: 0388-1350 ISO Abbreviation: J Toxicol Sci Publication Date: 1993 Aug |
Date Detail:
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Created Date: 1994-01-05 Completed Date: 1994-01-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7805798 Medline TA: J Toxicol Sci Country: JAPAN |
Other Details:
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Languages: eng Pagination: 143-53 Citation Subset: IM |
Affiliation:
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Department of Hygiene, Sapporo Medical College, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aflatoxin B1
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toxicity Animals Aspartame / toxicity Canavanine / toxicity Cattle Cell Aging / drug effects* Cell Division Cells, Cultured / drug effects, enzymology Fibroblasts / drug effects Food Analysis* Glucosephosphate Dehydrogenase / metabolism Humans Isothiocyanates / toxicity Tetrazolium Salts Thiazoles |
| Chemical | |
Reg. No./Substance:
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0/Isothiocyanates; 0/Tetrazolium Salts; 0/Thiazoles; 1162-65-8/Aflatoxin B1; 22839-47-0/Aspartame; 298-93-1/thiazolyl blue; 543-38-4/Canavanine; 57-06-7/allyl isothiocyanate; EC 1.1.1.49/Glucosephosphate Dehydrogenase |
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