Document Detail


The effect of flow on vascular endothelial cells grown in tissue culture on polytetrafluoroethylene grafts.
MedLine Citation:
PMID:  3082028     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vascular grafts lined with endothelial cells (EC) grown to confluence in culture before implantation may provide a thromboresistant flow surface. Growth of EC on and their adherence to currently available prosthetic materials under conditions of flow are two impediments remaining in the development of such a graft. To address these problems, 22 polytetrafluoroethylene grafts (PTFE) (5 cm by 4 mm inside diameter) were pretreated with collagen and fibronectin, seeded with 2 to 3 X 10(6) bovine aortic EC per graft, and placed in tissue culture (seeded grafts). Twenty-two grafts pretreated with collagen and fibronectin alone served as controls. After 2 weeks morphologic studies revealed that 20/22 seeded grafts were lined with a confluent endothelial layer. Indium 111-oxine was then used to label the EC-seeded grafts. After exposure to either low (25 ml/min) or high (200 ml/min) flow rates for 60 minutes in an in vitro circuit, examination of the luminal surface of the graft by light microscopy and scanning electron microscopy revealed minimal loss of EC. These findings were corroborated by radionuclide scans that showed an insignificant loss of the EC-associated indium label during exposure to flow (7% low flow, 11% high flow). Pretreatment of PTFE grafts with collagen and fibronectin thus promotes both attachment and adherence of EC even under flow conditions.
Authors:
J M Sentissi; K Ramberg; T F O'Donnell; R J Connolly; A D Callow
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Surgery     Volume:  99     ISSN:  0039-6060     ISO Abbreviation:  Surgery     Publication Date:  1986 Mar 
Date Detail:
Created Date:  1986-04-04     Completed Date:  1986-04-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0417347     Medline TA:  Surgery     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  337-43     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Aorta / cytology*
Blood Vessel Prosthesis*
Collagen
Culture Techniques
Endothelium / cytology
Fibronectins
Indium / diagnostic use
Microscopy, Electron, Scanning
Microscopy, Phase-Contrast
Organometallic Compounds*
Oxyquinoline / analogs & derivatives,  diagnostic use
Polytetrafluoroethylene*
Radioisotopes / diagnostic use
Regional Blood Flow
Rheology
Grant Support
ID/Acronym/Agency:
H1R01 HL28855/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Fibronectins; 0/Organometallic Compounds; 0/Radioisotopes; 14514-42-2/indium oxine; 148-24-3/Oxyquinoline; 7440-74-6/Indium; 9002-84-0/Polytetrafluoroethylene; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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