| The effect of the extracellular matrix on the detachment of human endothelial cells. | |
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MedLine Citation:
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PMID: 6094597 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human umbilical vein endothelial cells can be serially passaged by supplementing medium with a partially purified growth factor. Cell-substratum detachment of early and late passage endothelial cells was examined using trypsin, collagenase, or homocysteine. Late-passage cells detached more rapidly than early passage cells under all conditions tested. The rate of detachment was dependent upon the specific agent used. Protease-mediated detachment was most rapid, occurring over minutes, in contrast to homocysteine-induced detachment, which occurred over hours. When detached cells were collected and replated in the absence of the detaching agent, these cells reattached, spread, and continued to proliferate. No significant difference was observed in the rate of adhesion of either early or late passage cells to a gelatin matrix. When early or late-passage endothelial cells were plated and grown to confluence on a matrix synthesized by the opposite cell type, the rate of protease-mediated cell detachment resembled the cell type from which the matrix was derived. The ease of endothelial cell detachment was determined by the origin of the extracellular matrix. Examination of the extracellular matrices from early and late passage cells revealed significant differences in the amounts of glycosaminoglycans and sulfated proteins present. These studies demonstrate the importance of the endothelial cell extracellular matrix in protease-mediated cell detachment. The rate of cell detachment was controlled by the extracellular matrices are not altered by the endothelial cells. |
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Authors:
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P B Gordon; M A Levitt; C S Jenkins; V B Hatcher |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of cellular physiology Volume: 121 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 1984 Dec |
Date Detail:
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Created Date: 1984-12-27 Completed Date: 1984-12-27 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 467-75 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Adhesion
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drug effects Culture Techniques Electrophoresis, Polyacrylamide Gel Endothelium / analysis, cytology Extracellular Matrix / physiology* Glycosaminoglycans / analysis Heparitin Sulfate / analysis Homocysteine / pharmacology Humans Infant, Newborn Microbial Collagenase / pharmacology Protein Biosynthesis Trypsin / pharmacology Umbilical Veins / cytology* |
| Grant Support | |
ID/Acronym/Agency:
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AG 01732/AG/NIA NIH HHS; HL 07080/HL/NHLBI NIH HHS; HL 16387/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Glycosaminoglycans; 454-28-4/Homocysteine; 9050-30-0/Heparitin Sulfate; EC 3.4.21.4/Trypsin; EC 3.4.24.3/Microbial Collagenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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