Document Detail


The effect of the extracellular matrix on the detachment of human endothelial cells.
MedLine Citation:
PMID:  6094597     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human umbilical vein endothelial cells can be serially passaged by supplementing medium with a partially purified growth factor. Cell-substratum detachment of early and late passage endothelial cells was examined using trypsin, collagenase, or homocysteine. Late-passage cells detached more rapidly than early passage cells under all conditions tested. The rate of detachment was dependent upon the specific agent used. Protease-mediated detachment was most rapid, occurring over minutes, in contrast to homocysteine-induced detachment, which occurred over hours. When detached cells were collected and replated in the absence of the detaching agent, these cells reattached, spread, and continued to proliferate. No significant difference was observed in the rate of adhesion of either early or late passage cells to a gelatin matrix. When early or late-passage endothelial cells were plated and grown to confluence on a matrix synthesized by the opposite cell type, the rate of protease-mediated cell detachment resembled the cell type from which the matrix was derived. The ease of endothelial cell detachment was determined by the origin of the extracellular matrix. Examination of the extracellular matrices from early and late passage cells revealed significant differences in the amounts of glycosaminoglycans and sulfated proteins present. These studies demonstrate the importance of the endothelial cell extracellular matrix in protease-mediated cell detachment. The rate of cell detachment was controlled by the extracellular matrices are not altered by the endothelial cells.
Authors:
P B Gordon; M A Levitt; C S Jenkins; V B Hatcher
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  121     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1984 Dec 
Date Detail:
Created Date:  1984-12-27     Completed Date:  1984-12-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  467-75     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Adhesion / drug effects
Culture Techniques
Electrophoresis, Polyacrylamide Gel
Endothelium / analysis,  cytology
Extracellular Matrix / physiology*
Glycosaminoglycans / analysis
Heparitin Sulfate / analysis
Homocysteine / pharmacology
Humans
Infant, Newborn
Microbial Collagenase / pharmacology
Protein Biosynthesis
Trypsin / pharmacology
Umbilical Veins / cytology*
Grant Support
ID/Acronym/Agency:
AG 01732/AG/NIA NIH HHS; HL 07080/HL/NHLBI NIH HHS; HL 16387/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Glycosaminoglycans; 454-28-4/Homocysteine; 9050-30-0/Heparitin Sulfate; EC 3.4.21.4/Trypsin; EC 3.4.24.3/Microbial Collagenase

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