Document Detail


The effect of exhaustive exercise on the antioxidant enzyme system in skeletal muscle from calcium-deficient rats.
MedLine Citation:
PMID:  9518504     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of the current study was to elucidate the synergism of dietary calcium restriction and exhaustive exercise in the antioxidant enzyme system of rat soleus muscle, and to investigate the involvement of neutrophils in exercise-induced muscle damage. Forty-eight male Wistar rats were assigned to the following groups: control (C) or calcium-restricted [1 month (1 M) or 3 months (3 M)]. Each group was subdivided into acutely exercised or non-exercised groups. Soleus muscle from each rat was analysed to determine the levels of antioxidant enzymes [Mn-superoxide dismutase (SOD), Cu, Zn-SOD, glutathione peroxidase (GPX), and catalase (CAT)]. Dietary calcium restriction resulted in calcium deficiency and upregulated the antioxidant enzymes examined except GPX. Conversely, exhaustive exercise significantly decreased GPX and CAT, but not SODs activities in the calcium-restricted (1 M and/or 3 M) rats. Contents of immunoreactive Mn-SOD and Cu,Zn-SOD were only increased in the 3 M rats. During calcium restriction, the mRNA expression of both forms of SOD showed initial upregulation, followed by downregulation. Exhaustive exercise significantly increased the mRNA expressions only in the 3 M rats. Moreover, exhaustive exercise markedly increased myeloperoxidase activity in soleus muscles from the 1 M and 3 M rats compared with the C rats, and significantly enhanced the ability of neutrophils to generate superoxide in the 3 M rats. The results demonstrate that dietary calcium restriction upregulates certain antioxidant enzyme activities in rat soleus muscle, indicating an enhanced resistance to potential increases in intracellular reactive oxygen species. The results also suggest that exhaustive exercise may cause oxidative damage in soleus muscle of calcium-deficient rats through the activation of neutrophils.
Authors:
S Ohishi; T Kizaki; T Ookawara; K Toshinai; S Haga; F Karasawa; T Satoh; N Nagata; L L Ji; H Ohno
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pflügers Archiv : European journal of physiology     Volume:  435     ISSN:  0031-6768     ISO Abbreviation:  Pflugers Arch.     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-06-08     Completed Date:  1998-06-08     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0154720     Medline TA:  Pflugers Arch     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  767-74     Citation Subset:  IM; S    
Affiliation:
Department of Hygiene, National Defense Medical College, 3-2, Namiki, Tokorozawa 359, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antioxidants / metabolism*
Body Weight
Calcium / blood,  deficiency*
Calcium, Dietary / administration & dosage
Catalase / metabolism
Glutathione Peroxidase / metabolism
Male
Muscle, Skeletal / anatomy & histology,  enzymology*
Neutrophils / metabolism
Organ Size
Peroxidase / metabolism
Phosphorus / blood
Physical Endurance
Physical Exertion / physiology*
RNA, Messenger / metabolism
Rats
Rats, Wistar
Superoxide Dismutase / genetics,  metabolism
Superoxides / metabolism
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Calcium, Dietary; 0/RNA, Messenger; 11062-77-4/Superoxides; 7440-70-2/Calcium; 7723-14-0/Phosphorus; EC 1.11.1.6/Catalase; EC 1.11.1.7/Peroxidase; EC 1.11.1.9/Glutathione Peroxidase; EC 1.15.1.1/Superoxide Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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