Document Detail

The effect of exercise and oxidant-antioxidant intervention on the levels of neurotrophins and free radicals in spinal cord of rats.
MedLine Citation:
PMID:  18936770     Owner:  NLM     Status:  MEDLINE    
STUDY DESIGN: This study was designed to investigate the effects of oxidant and antioxidant treatment, as well as regular exercise, on neurotrophin levels in the spinal cord of rats.
OBJECTIVES: Reactive oxygen species (ROS) play a role in neurodegenerative diseases, but ROS at moderate levels could stimulate biochemical processes through redox-sensitive transcription.
METHODS: Exercised or sedentary animals were injected subcutaneously with hydrogen peroxide (H(2)O(2)), N-tert butyl-alpha-phenyl nitrone (PBN) or saline for the last 2 weeks of a 10-week experimental period to challenge redox balance. Free radical (FR) concentration was evaluated in the spinal cord by electron spin resonance, protein carbonyls, brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF) levels and the mRNA expression of BDNF receptor and tyrosine kinase receptor B (TrKB).
SETTING: Research Institute of Sport Science, Semmelweis University, Budapest, Hungary.
RESULTS: Exercise or PBN decreased the concentration of FR, whereas the carbonyl content did not change. BDNF was significantly decreased in exercised sham and sedentary PBN-treated groups, and its content correlated with the level of FR. GDNF was significantly increased in sedentary H(2)O(2)-treated groups. No differences were observed in TrkB mRNA expression among groups.
CONCLUSIONS: Results suggest that regular exercise alone and PBN in sedentary animals can successfully decrease FR levels in the spinal cord. Redox alteration seems to affect the levels of GDNF and BDNF, which might have clinical consequences, as neurotrophins play an important role in cellular resistance and regeneration.
S Siamilis; J Jakus; C Nyakas; A Costa; B Mihalik; A Falus; Z Radak
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-21
Journal Detail:
Title:  Spinal cord     Volume:  47     ISSN:  1476-5624     ISO Abbreviation:  Spinal Cord     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-06-08     Completed Date:  2009-09-01     Revised Date:  2011-06-06    
Medline Journal Info:
Nlm Unique ID:  9609749     Medline TA:  Spinal Cord     Country:  England    
Other Details:
Languages:  eng     Pagination:  453-7     Citation Subset:  IM    
Faculty of Physical Education and Sport Science, Institute of Sport Science, Semmelweis University, Budapest, Hungary.
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MeSH Terms
Analysis of Variance
Antioxidants / pharmacology*
Brain-Derived Neurotrophic Factor / genetics,  metabolism
Cyclic N-Oxides / pharmacology
Free Radicals / metabolism
Gene Expression Regulation / drug effects
Glial Cell Line-Derived Neurotrophic Factor / genetics,  metabolism
Hydrogen Peroxide / pharmacology
Nerve Growth Factors / classification,  genetics,  metabolism*
Oxidants / pharmacology*
Physical Conditioning, Animal / methods*
Protein Carbonylation / drug effects
RNA, Messenger / metabolism
Rats, Wistar
Spinal Cord / drug effects*,  metabolism*
Reg. No./Substance:
0/Antioxidants; 0/Brain-Derived Neurotrophic Factor; 0/Cyclic N-Oxides; 0/Free Radicals; 0/Glial Cell Line-Derived Neurotrophic Factor; 0/Nerve Growth Factors; 0/Oxidants; 0/RNA, Messenger; 3376-24-7/phenyl-N-tert-butylnitrone; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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