Document Detail

The effect of ethanol upon early development in mice and rats. XXI. The effect of acute maternal ethanol intoxication upon in vitro hatching of mouse embryos.
MedLine Citation:
PMID:  8680019     Owner:  NLM     Status:  MEDLINE    
The effect of acute ethanol intoxication (ethanol 33% in a.d.) in mice on day 4 of pregnancy (at 9 o'clock a.m.) upon in vitro hatching has been investigated. The embryos were cultured in modified Brinster medium (1963), at 37 degrees C and 5% CO2 in air beginning at 2 o'clock p.m. on day 4 of pregnancy. The control of the developmental stage of embryos from experimental and control groups on day 4 of pregnancy (at 9 o'clock a.m.) showed some numerical differences between the embryos found in early and mid blastocyst stages but no difference between advanced blastocysts. The control after 24 hours of culture showed in both experimental and control groups the presence of expanded blastocysts. No hatched or degenerated embryos were observed. The control at 48 hours revealed the following aspects: the inhibition of hatching revealed by a significantly lower percentage of in vitro hatching (16.3%) in the experimental as compared with the control group (36.6%); the percentage of degenerated embryos is significantly increased in the ethanol group (13%) as compared with the untreated group (6.10%); the percentage of embryos with the zona pellucida fractured does not differ significantly between experimental and control groups. Some problems related to the mechanism of in vitro and in vivo, hatching are discussed.
M Checiu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie     Volume:  41     ISSN:  1220-0522     ISO Abbreviation:  Rom J Morphol Embryol     Publication Date:    1995 Jan-Jun
Date Detail:
Created Date:  1996-08-21     Completed Date:  1996-08-21     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9112454     Medline TA:  Rom J Morphol Embryol     Country:  ROMANIA    
Other Details:
Languages:  eng     Pagination:  13-8     Citation Subset:  IM    
Laboratory of Embryology and Teratology, Center of Medicine and Sanitary Services and Management, Timişoara, Romania.
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MeSH Terms
Alcoholic Intoxication / complications*
Blastocyst / drug effects,  physiology*
Culture Techniques
Embryonic and Fetal Development
Ethanol / pharmacology
Maternal-Fetal Exchange
Zona Pellucida / physiology
Reg. No./Substance:

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