Document Detail


The effect of estradiol-17 beta and actinomycin D on the release of PGF and PGE from separated cells of human endometrium.
MedLine Citation:
PMID:  3432558     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Estradiol-17 beta selectively stimulated the release of PGF from separated glandular but not stromal cells of human secretory endometrium (p less than 0.025) but had no effect on PGF release from either type of cells obtained from proliferative endometrium. PGE release was not affected by estradiol-17 beta. Actinomycin D did not antagonise the effect of estradiol-17 beta on PGF release from secretory, glandular cells. Basal release of PGF from these cells was stimulated by actinomycin D alone (100 ng/ml) (p less than 0.025) and PGE release stimulated in the presence of estradiol-17 beta. Actinomycin D had no effect on PGF or PGE release from proliferative endometrium. These findings suggest that estradiol-17 beta stimulates PGF release by a mechanism that does not affect PGE release and which is not dependent on the synthesis of new protein. The basal release of PGF and PGE by glandular cells of secretory endometrium in vitro is regulated by protein/proteins which reduce PG release.
Authors:
S K Smith; R W Kelly
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Prostaglandins     Volume:  34     ISSN:  0090-6980     ISO Abbreviation:  Prostaglandins     Publication Date:  1987 Oct 
Date Detail:
Created Date:  1988-03-14     Completed Date:  1988-03-14     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0320271     Medline TA:  Prostaglandins     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  553-61     Citation Subset:  IM    
Affiliation:
MRC Reproductive Biology Unit, Centre for Reproductive Biology, Edinburgh.
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MeSH Terms
Descriptor/Qualifier:
Cell Separation
Cells, Cultured
Dactinomycin / pharmacology*
Endometrium / cytology,  drug effects*,  metabolism
Estradiol / pharmacology*
Female
Humans
Prostaglandins E / metabolism*
Prostaglandins F / metabolism*
Chemical
Reg. No./Substance:
0/Prostaglandins E; 0/Prostaglandins F; 50-28-2/Estradiol; 50-76-0/Dactinomycin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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