| The effect of an endogenous Na+, K+-ATPase inhibitor on rat lens transparency and ultrastructure. | |
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MedLine Citation:
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PMID: 12735627 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. The purpose of the present study was to analyze the possible effect of ouabain and an endogenous ouabain-like substance (endobain E), on lenses of 100- and 400-g body weight rats. 2. Lenses were incubated with ouabain or endobain E for 120 min, either at room temperature or in the cold; opalescence was checked by gross examination and ultrastructure by electron microscopy. 3. Lenses from 400-g rats invariably remained translucent whereas those from 100-g rats presented variable opalescence. 4. As disclosed with the electron microscope, lenses of 100-g rats incubated at room temperature, with or without ouabain or endobain E, presented variable degrees of ultrastructural changes: with ouabain, there was fiber separation and vacuole formation but with endobain E, no vacuoles were found and fibers, though disorganized, appeared attached. After incubation in an ice bath, lenses were markedly altered in all conditions assayed. 5. It is concluded that ouabain and endobain E effect on lens transparency depends on the rat age and that in young animals, it is crucial incubation temperature during experimental procedure. |
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Authors:
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Amanda Pellegrino de Iraldi; Clara Peña; Georgina Rodríguez de Lores Arnaiz |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cellular and molecular neurobiology Volume: 23 ISSN: 0272-4340 ISO Abbreviation: Cell. Mol. Neurobiol. Publication Date: 2003 Apr |
Date Detail:
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Created Date: 2003-05-08 Completed Date: 2003-12-04 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8200709 Medline TA: Cell Mol Neurobiol Country: United States |
Other Details:
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Languages: eng Pagination: 131-41 Citation Subset: IM |
Affiliation:
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Instituto de Biología Celular y Neurociencias "Prof E. De Robertis," Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Buenos Aires, Argentina. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Age Factors Animals Enzyme Inhibitors / pharmacology* Lens, Crystalline / drug effects*, enzymology, ultrastructure* Male Ouabain / analogs & derivatives*, pharmacology* Rats Rats, Wistar Sodium-Potassium-Exchanging ATPase / antagonists & inhibitors*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/endobain E; 630-60-4/Ouabain; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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