Document Detail

The effect of elevated temperature on the aggregation of African green monkey kidney cells.
MedLine Citation:
PMID:  3401937     Owner:  NLM     Status:  MEDLINE    
The aggregation kinetics of African green monkey kidney cells CV1 and of the SV40 transformed derivative COS1 cells that had been incubated at 37 degrees C or 43.5 degrees C was studied using the shaking flask system. COS1 cells show a three fold decrease in aggregation rate compared to CV1 cells when both cell types were incubated and aggregated at 37 degrees C. When these cell types were incubated at 43.5 degrees C for 5 hours, then aggregated at 37 degrees C showed a faster aggregation kinetics than before. Their aggregation at 43.5 degrees C with prior incubation at 37 degrees C or 43.5 degrees C reached the aggregation kinetics of 43.5 degrees C incubated cells aggregated at 37 degrees C. The addition of serum in the aggregation medium did not influence extensively the aggregation rates of both cell types.
D P Matthopoulos; M Tzaphlidou
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell biology international reports     Volume:  12     ISSN:  0309-1651     ISO Abbreviation:  Cell Biol. Int. Rep.     Publication Date:  1988 May 
Date Detail:
Created Date:  1988-09-12     Completed Date:  1988-09-12     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7708050     Medline TA:  Cell Biol Int Rep     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  413-24     Citation Subset:  IM    
Laboratory of General Biology, School of Medicine, University of Ioannina, Greece.
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MeSH Terms
Body Temperature
Cell Aggregation*
Cell Line
Cell Line, Transformed
Cercopithecus aethiops
Culture Media
Kidney / cytology*
Reg. No./Substance:
0/Culture Media

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