Document Detail


The effect of an electrically conductive carbon nanotube/collagen composite on neurite outgrowth of PC12 cells.
MedLine Citation:
PMID:  20665676     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report the preparation of an electrically conductive composite composed of collagen and carbon nanotubes (CNTs) and its use as a substrate for the in vitro growth of PC12 cells. Morphological observation by scanning electron microscopy (SEM) indicated the homogenous dispersion of CNTs in the collagen matrix. Four-point probe and cyclic voltammogram studies demonstrated the enhanced electroactivity and a lowered electrical resistivity of the resulting composites even at low loadings (<5%) of CNTs. Cellular metabolic activity was evaluated by the MTT assay. Cell viability was systematically related to the amount of CNTs embedded in the collagen matrix. SEM and immunofluorescent images have indicated that the morphological features of PC12 cells were dominantly influenced by electrical potential. Greater neurite extension was preferentially induced on the exposure of electrical stimulation by facilitating the differentiation of PC12 cells into neurons indicated by more significant filopodium extension. These electrically conductive, biocompatible CNT/collagen composites could be of benefit for the development of novel neural electrodes, enhancing the growth, differentiation, and branching of neurons in an electrically driven way.
Authors:
Youngnam Cho; Richard Ben Borgens
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  95     ISSN:  1552-4965     ISO Abbreviation:  J Biomed Mater Res A     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-09-22     Completed Date:  2011-01-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  510-7     Citation Subset:  IM    
Affiliation:
Center for Paralysis Research, School of Veterinary Medicine, Purdue University, West Lafayette, Indiana, USA. cho22@purdue.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Biocompatible Materials / chemistry,  metabolism
Cell Proliferation
Cell Survival
Collagen / chemistry,  pharmacology*
Electric Conductivity
Materials Testing
Nanotubes, Carbon / chemistry*
Neurites / drug effects*,  metabolism,  ultrastructure
PC12 Cells* / cytology,  drug effects
Rats
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Nanotubes, Carbon; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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