Document Detail


The effect of the elapsed time between blood draw and processing on the recovery of fetal cells from maternal blood.
MedLine Citation:
PMID:  15051035     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To test the hypothesis that a delay in initial fetal cell enrichment processing of maternal blood samples (defined as the time between blood draw and the initial density gradient centrifugation step) compromises the ability to recover fetal cells, we performed a randomized comparison of immediate (within 4 hours of draw) versus delayed (between 18-24 hours of draw) processing. METHODS: Four centers participated: two centers utilized flow cytometry (FLOW), and two centers utilized magnetic-activated cell sorting (MACS) techniques. Each center collected 34 samples. The outcome was the percentage of gamma positive (gamma(+)) cells for FLOW or the number of nucleated red blood cells (NRBCs) for MACS, found in the final enriched cell population. Both outcomes reflect cell properties that are potentially fetal in origin, thus making them representative of the ability to recover fetal cells. RESULTS: Our results did not support our hypothesis that delay in processing compromises fetal cell recovery. Instead, in MACS processing, we observed an increase in recovered NRBCs when blood sample processing was delayed compared with immediate processing. There was no significant difference in gamma(+) cells with FLOW. CONCLUSION: Time-related changes in the density of target cells, perhaps associated with their progress towards apoptosis during the delay period, may result in increased intact fetal cells with the study methods utilized.
Authors:
K A Dukes; L M Sullivan; D Lewis; K L Johnson; D W Bianchi; J L Simpson; W Holzgreve; S Hahn; F Z Bischoff; L G Jackson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of the Society for Gynecologic Investigation     Volume:  11     ISSN:  1071-5576     ISO Abbreviation:  J. Soc. Gynecol. Investig.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-03-30     Completed Date:  2004-11-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9433806     Medline TA:  J Soc Gynecol Investig     Country:  United States    
Other Details:
Languages:  eng     Pagination:  154-65     Citation Subset:  IM    
Affiliation:
DM-STAT Inc., Medford, Massachusetts 02155, USA. Kim.Dukes@dmstat.com
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MeSH Terms
Descriptor/Qualifier:
Apoptosis
Blood Specimen Collection / methods*
Cell Count
Cell Separation / methods
Centrifugation, Density Gradient
Erythroblasts
Female
Fetal Blood / cytology*
Flow Cytometry
Humans
Magnetics
Pregnancy
Time Factors
Grant Support
ID/Acronym/Agency:
N01-HD-4-3204/HD/NICHD NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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