Document Detail


The effect of eicosapentaenoic acid on rat lymphocyte proliferation depends upon its position in dietary triacylglycerols.
MedLine Citation:
PMID:  14652377     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Animal and human studies have shown that greatly increasing the amount of fish oil [rich in long-chain (n-3) PUFA] in the diet can decrease lymphocyte functions. The effects of a more modest provision of long-chain (n-3) PUFA and whether eicosapentaenoic acid (20:5) and docosahexaenoic acid (22:6) have the same effects as one another are unclear. Whether the position of 20:5 or 22:6 in dietary triacylglycerols (TAG) influences their incorporation into immune cells and their subsequent functional effects is not known. In this study, male weanling rats were fed for 6 wk one of 9 diets that contained 178 g lipid/kg and that differed in the type of (n-3) PUFA and in the position of these in dietary TAG. The control diet contained 4.4 g alpha-linolenic acid (18:3)/100 g total fatty acids. In the other diets, 20:5 or 22:6 replaced a portion (50 or 100%) of 18:3, and were in the sn-2 or the sn-1(3) position of dietary TAG. There were significant dose-dependent increases in the proportion of 20:5 or 22:6 in spleen mononuclear cell phospholipids when 20:5 or 22:6 was fed. These increases were at the expense of arachidonic acid and were largely independent of the position of 20:5 or 22:6 in dietary TAG. Spleen lymphocyte proliferation increased dose dependently when 20:5 was fed in the sn-1(3) position of dietary TAG. There were no significant differences in interleukin-2, interferon-gamma or interleukin-10 production among spleen cells from rats fed the different diets. Prostaglandin E(2) production by spleen mononuclear cells was decreased by inclusion of either 20:5 or 22:6 in the diet in the sn-1(3) position. Thus, incorporation of 20:5 or 22:6 into spleen mononuclear cell phospholipids is not influenced by the position in dietary TAG. However, the pattern of incorporation may be influenced, and there are some differential functional effects of the position of long-chain (n-3) PUFA in dietary TAG. A moderate increase in the intake of 20:5 at the sn-1(3) position of dietary TAG increases lymphocyte proliferation.
Authors:
S Kew; S Wells; F Thies; G P McNeill; P T Quinlan; G T Clark; H Dombrowsky; A D Postle; P C Calder
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of nutrition     Volume:  133     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-12-03     Completed Date:  2004-01-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4230-8     Citation Subset:  IM    
Affiliation:
Institute of Human Nutrition, Division of Infection, Inflammation and Repair, University of Southampton, Southampton, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Weight
Cell Division / drug effects
Cells, Cultured
Concanavalin A / pharmacology
Cytokines / metabolism
Diet
Eicosapentaenoic Acid / chemistry*,  pharmacology*
Fatty Acids / metabolism
Leukocytes, Mononuclear / metabolism
Lymphocytes / cytology*
Lymphoid Tissue / anatomy & histology
Male
Molecular Structure
Organ Size
Phospholipids / metabolism
Rats
Rats, Inbred Lew
Spleen / cytology,  metabolism
Triglycerides / administration & dosage*,  chemistry*
Chemical
Reg. No./Substance:
0/Cytokines; 0/Fatty Acids; 0/Phospholipids; 0/Triglycerides; 11028-71-0/Concanavalin A; 1553-41-9/Eicosapentaenoic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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