Document Detail


The effect of diet and cardiovascular risk on ovarian aging in cynomolgus monkeys (Macaca fascicularis).
MedLine Citation:
PMID:  20458254     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The aim of this study was to determine the relationships among dietary protein source, cardiovascular risk, reproductive hormones, and ovarian aging.
METHODS: Adult female cynomolgus monkeys (Macaca fascicularis) were assigned randomly to one of two diets containing saturated fat and cholesterol, differing only by protein source: (1) casein-lactalbumin (n = 29) or (2) soy protein with isoflavones (n = 32). Cardiovascular risk markers and reproductive hormones were measured at baseline and after 32 months of treatment, at which time the ovaries were removed and serially sectioned and ovarian follicles were counted in every 100th section.
RESULTS: Casein-lactalbumin-fed monkeys had fewer primordial, primary, and secondary follicles (all P values < 0.05) than did their soy-fed counterparts. Antimüllerian hormone was significantly correlated with all follicle types (r values > or = 0.66, P < 0.001) for casein-fed monkeys and was significantly correlated with primary (rsoy = 0.47, P = 0.005) and secondary (rsoy = 0.45, P = 0.007) follicles in soy-fed monkeys. No significant associations were seen between any of the other reproductive hormones measured and follicle counts. Casein-lactalbumin-fed monkeys had a more atherogenic lipoprotein profile and increased atherosclerosis extent (P < 0.05), but despite these differences in cardiovascular risk between monkeys fed with casein-lactalbumin and monkeys fed with soy, none of the individual cardiovascular risk markers measured in this study explained the relationship between dietary protein source and follicle counts (linear regression, all P values > 0.05).
CONCLUSIONS: Diet influences the rate of follicular depletion in cynomolgus macaques; however, the mechanism for this effect remains undetermined.
Authors:
Susan E Appt; Haiying Chen; Amanda K Goode; Patricia B Hoyer; Thomas B Clarkson; Michael R Adams; Mark E Wilson; Adrian A Franke; Jay R Kaplan
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Menopause (New York, N.Y.)     Volume:  17     ISSN:  1530-0374     ISO Abbreviation:  Menopause     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-09     Completed Date:  2010-11-01     Revised Date:  2011-09-26    
Medline Journal Info:
Nlm Unique ID:  9433353     Medline TA:  Menopause     Country:  United States    
Other Details:
Languages:  eng     Pagination:  741-8     Citation Subset:  IM    
Affiliation:
Department of Pathology/Comparative Medicine, Wake Forest University School of Medicine, Winston-Salem, NC, USA. sappt@wfubmc.edu
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MeSH Terms
Descriptor/Qualifier:
Aging / physiology
Androstenedione / blood
Animals
Anti-Mullerian Hormone / blood
Atherosclerosis / pathology*
C-Reactive Protein / analysis
Caseins / administration & dosage*
Chemokine CCL2 / blood
Diet
Estradiol / blood
Female
Iliac Artery / pathology
Interleukin-6 / blood
Isoflavones / administration & dosage*,  blood
Lactalbumin / administration & dosage*
Lipids / blood*
Macaca fascicularis
Ovarian Follicle / pathology*,  physiology
Ovariectomy
Random Allocation
Retrospective Studies
Soybean Proteins / administration & dosage*
Testosterone / blood
Grant Support
ID/Acronym/Agency:
ES06694/ES/NIEHS NIH HHS; R01 AG021948/AG/NIA NIH HHS; R01 AG021948-05/AG/NIA NIH HHS; R01 AG027847/AG/NIA NIH HHS; R01 AG027847-04/AG/NIA NIH HHS; R01 AG027847-06/AG/NIA NIH HHS; R01 HL0794/HL/NHLBI NIH HHS; R01 HL079421-05/HL/NHLBI NIH HHS; R24 RR022191/RR/NCRR NIH HHS; R24 RR022191-04/RR/NCRR NIH HHS; S10 RR020890/RR/NCRR NIH HHS; S10 RR020890-010004/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Caseins; 0/Chemokine CCL2; 0/Interleukin-6; 0/Isoflavones; 0/Lipids; 0/Soybean Proteins; 50-28-2/Estradiol; 58-22-0/Testosterone; 63-05-8/Androstenedione; 80497-65-0/Anti-Mullerian Hormone; 9007-41-4/C-Reactive Protein; 9013-90-5/Lactalbumin
Comments/Corrections

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