Document Detail

The effect of dibutyryl cyclic AMP and butyrate on F9 teratocarcinoma cellular retinoic acid-binding protein activity.
MedLine Citation:
PMID:  3031026     Owner:  NLM     Status:  MEDLINE    
F9 teratocarcinoma cells contain a cellular retinoic acid-binding protein (CRABP) that may mediate the retinoic acid-induced differentiation of this cell line. Specific [3H]retinoic acid binding to CRABP in F9 stem cell cytosol is protein-dependent, reaches equilibrium within 4 h at 4 degrees C, and yields 643 +/- 105 fmol of [3H]retinoic acid per mg of protein with an apparent dissociation constant of 9.2 +/- 1.1 nM. When F9 stem cells are grown in the presence of either dibutyryl cyclic AMP or sodium butyrate, CRABP activity is stimulated 2-4-fold. The effect of these drugs on CRABP activity is both time and concentration-dependent, resulting in an increase in the number of binding sites for [3H]retinoic acid with no change in their affinity. The new [3H]retinoic acid-binding sites have a sedimentation coefficient of 2 S and are not displaced by excess retinol. When F9 stem cells are grown in the presence of cyclic 8-bromo-AMP or cholera toxin, no increase in CRABP activity is observed. We conclude that the stimulation of CRABP activity by dibutyryl cyclic AMP may result from the action of butyrate. In addition, the stimulation of retinoic acid-induced F9 cell differentiation by cyclic AMP analogs (Strickland, S., Smith, K.K., and Marotti, K.R. (1980) Cell 21, 347-355) and the inhibition of this differentiation by butyrate (Levine R. A., Campisi, J., Wang, S.-Y., and Gudas, L. J. (1984) Dev. Biol. 105, 443-450) are not correlated with increases or decreases, respectively, in the level of CRABP activity.
J F Grippo; L J Gudas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  262     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1987 Apr 
Date Detail:
Created Date:  1987-05-15     Completed Date:  1987-05-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4492-500     Citation Subset:  IM    
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MeSH Terms
Bucladesine / pharmacology*
Butyrates / pharmacology*
Butyric Acid
Butyric Acids / pharmacology*
Carrier Proteins / metabolism*
Cell Differentiation / drug effects
Cell Line
Cyclic AMP / metabolism
Cytosol / metabolism
Receptors, Retinoic Acid
Teratoma / metabolism*,  pathology
Grant Support
1 F32 CA07971-01/CA/NCI NIH HHS; 1T32 CA09361/CA/NCI NIH HHS; P01 CA22427/CA/NCI NIH HHS
Reg. No./Substance:
0/Butyrates; 0/Butyric Acids; 0/Carrier Proteins; 0/Receptors, Retinoic Acid; 107-92-6/Butyric Acid; 362-74-3/Bucladesine; 60-92-4/Cyclic AMP

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