Document Detail


An effect of dexamethasone on adenosine 3',5'-monophosphate content and adenosine 3',5'-monophosphate phosphodiesterase activity of cultured hepatoma cells.
MedLine Citation:
PMID:  4341439     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of dexamethasone on adenosine 3',5'-monophosphate (cAMP) phosphodiesterase activity in cultured HTC hepatoma cells was investigated. Homogenates of these cells contain phosphodiesterase activity with two apparent Michaelis constants for cAMP (2-5 mum and 50 mum). At all substrate concentrations tested, phosphodiesterase activity was decreased 25-40% in cells incubated for 36 hr or more with 1 mum dexamethasone. Acid phosphatase activity in the same cells was not decreased. alpha-Methyl testosterone, 1 mum, was without effect on phosphodiesterase activity. Incubation for 10 min with epinephrine plus theophylline increased the cAMP content of the HTC cells 3- to 6-fold. In cells incubated for 72 hr with dexamethasone, the basal concentration of cAMP was slightly increased and the increment produced by epinephrine plus theophylline was markedly increased. We believe that in many cells the so-called permissive effects of steroid hormones on cAMP mediated processes may be due to an effect of these hormones on cAMP phosphodiesterase activity similar to that observed in HTC cells incubated with dexamethasone.
Authors:
V Manganiello; M Vaughan
Related Documents :
9746789 - Type 4 cyclic adenosine monophosphate phosphodiesterase as a therapeutic target in chro...
20194589 - Cdpa is a burkholderia pseudomallei cyclic di-gmp phosphodiesterase involved in autoagg...
3422429 - Isolation of 1-monomethylphosphoinositol 4,5-bisphosphate [a product of methanolysis of...
2987469 - The effects of calcium regulating hormones on bone resorption by isolated human osteocl...
187799 - Transport, phosphorylation, and toxicity of a tricyclic nucleoside in cultured novikoff...
8033589 - High affinity vasoactive intestinal peptide receptors on fetal human nonpigmented cilia...
17652349 - The pax-3 gene is involved in vulva formation in pristionchus pacificus and is a target...
4077699 - Quantitative distribution of enteroendocrine cells in the gastrointestinal tract of the...
18710819 - Hypoxia induces resistance to 5-fluorouracil in oral cancer cells via g(1) phase cell c...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  51     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  1972 Oct 
Date Detail:
Created Date:  1972-10-25     Completed Date:  1972-10-25     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2763-7     Citation Subset:  AIM; IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / analysis
Carcinoma, Hepatocellular / enzymology,  metabolism*
Cells, Cultured
Cyclic AMP / metabolism*
Dexamethasone / pharmacology*
Epinephrine / pharmacology
Kinetics
Liver Neoplasms
Methyltestosterone / pharmacology
Phosphodiesterase Inhibitors
Phosphoric Monoester Hydrolases / antagonists & inhibitors*
Theophylline / pharmacology
Tritium
Chemical
Reg. No./Substance:
0/Phosphodiesterase Inhibitors; 10028-17-8/Tritium; 50-02-2/Dexamethasone; 51-43-4/Epinephrine; 58-18-4/Methyltestosterone; 58-55-9/Theophylline; 60-92-4/Cyclic AMP; EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC 3.1.3.2/Acid Phosphatase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Critical role of the carbohydrate side chains of collagen in platelet aggregation.
Next Document:  A classification of the murine leukemia viruses. Neutralization of pseudotypes of Friend spleen focu...