Document Detail

An effect of dexamethasone on adenosine 3',5'-monophosphate content and adenosine 3',5'-monophosphate phosphodiesterase activity of cultured hepatoma cells.
MedLine Citation:
PMID:  4341439     Owner:  NLM     Status:  MEDLINE    
The effect of dexamethasone on adenosine 3',5'-monophosphate (cAMP) phosphodiesterase activity in cultured HTC hepatoma cells was investigated. Homogenates of these cells contain phosphodiesterase activity with two apparent Michaelis constants for cAMP (2-5 mum and 50 mum). At all substrate concentrations tested, phosphodiesterase activity was decreased 25-40% in cells incubated for 36 hr or more with 1 mum dexamethasone. Acid phosphatase activity in the same cells was not decreased. alpha-Methyl testosterone, 1 mum, was without effect on phosphodiesterase activity. Incubation for 10 min with epinephrine plus theophylline increased the cAMP content of the HTC cells 3- to 6-fold. In cells incubated for 72 hr with dexamethasone, the basal concentration of cAMP was slightly increased and the increment produced by epinephrine plus theophylline was markedly increased. We believe that in many cells the so-called permissive effects of steroid hormones on cAMP mediated processes may be due to an effect of these hormones on cAMP phosphodiesterase activity similar to that observed in HTC cells incubated with dexamethasone.
V Manganiello; M Vaughan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  51     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  1972 Oct 
Date Detail:
Created Date:  1972-10-25     Completed Date:  1972-10-25     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2763-7     Citation Subset:  AIM; IM    
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MeSH Terms
Acid Phosphatase / analysis
Carcinoma, Hepatocellular / enzymology,  metabolism*
Cells, Cultured
Cyclic AMP / metabolism*
Dexamethasone / pharmacology*
Epinephrine / pharmacology
Liver Neoplasms
Methyltestosterone / pharmacology
Phosphodiesterase Inhibitors
Phosphoric Monoester Hydrolases / antagonists & inhibitors*
Theophylline / pharmacology
Reg. No./Substance:
0/Phosphodiesterase Inhibitors; 10028-17-8/Tritium; 50-02-2/Dexamethasone; 51-43-4/Epinephrine; 58-18-4/Methyltestosterone; 58-55-9/Theophylline; 60-92-4/Cyclic AMP; EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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