| The effect of delta-aminolevulinate on catalase T-messenger RNA levels in delta-aminolevulinate synthase-defective mutants of Saccharomyces cerevisiae. | |
| | |
MedLine Citation:
|
PMID: 6997287 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Total RNA was isolated from mutants of Saccharomyces cerevisiae that lack active delta-aminolevulinate synthase and are therefore defective in heme biosynthesis. The RNAs were translated in the cell-free protein synthesis system from wheat germ, and the catalase T synthesized was isolated by immunoadsorption and polyacrylamide gel electrophoresis in the presence of dodecyl sulfate. Little or no catalase T product was detected with RNA from mutant cells grown in the absence of a heme precursor. As judged from in vitro translational capacity, RNA fractions from mutant cells grown in the presence of delta-aminolevulinate contained at least 10 times more catalase T mRNA than RNA from unsupplemented cells. |
| | |
Authors:
|
K Richter; G Ammerer; E Hartter; H Ruis |
Related Documents
:
|
6440787 - Localization of ornithine decarboxylase in mutant cho cells that overproduce the enzyme... 19252527 - Rnase l downmodulation of the rna-binding protein, hur, and cellular growth. 667167 - Rna metabolism in m1 adrenergic neuroblastoma cells. 18253947 - Cell (a549)-particle (jasada bhasma) interactions using raman spectroscopy. 6311197 - Leukotrienes stimulate acid secretion from isolated gastric parietal cells. 7880167 - Stochastic resonance as a possible mechanism of amplification of weak electric signals ... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 255 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1980 Sep |
Date Detail:
|
Created Date: 1980-11-20 Completed Date: 1980-11-20 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 8019-22 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
5-Aminolevulinate Synthetase
/
genetics* Aminolevulinic Acid / pharmacology* Catalase / biosynthesis* Heme / biosynthesis Levulinic Acids / pharmacology* Mutation Plants / metabolism Protein Biosynthesis / drug effects RNA, Messenger / metabolism* Saccharomyces cerevisiae / drug effects, enzymology*, genetics Triticum / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Levulinic Acids; 0/RNA, Messenger; 106-60-5/Aminolevulinic Acid; 14875-96-8/Heme; EC 1.11.1.6/Catalase; EC 2.3.1.37/5-Aminolevulinate Synthetase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Receptor mediated insulin degradation decreased by chloroquine in isolated rat adipocytes.
Next Document: On the mechanism of ribonucleoside diphosphate reductase from Escherichia coli. Evidence for 3'-C--H...