| The effect of cytotoxic lymphocytes on contraction, action potential and calcium handling in cultured myocardial cells. | |
| | |
MedLine Citation:
|
PMID: 8540399 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cytotoxic T lymphocytes are important in the pathogenesis of several disease states, yet the pathophysiology of the lymphocyte-myocyte interaction is not well known. We have developed in vitro viral and autoimmune models to study the physiological phenomena associated with this interaction. To produce these models, lymphocytes were obtained from adult rats injected either with mengo virus or autologous cardiac myocytes. Cardiac myocytes from neonatal rats were then exposed to these lymphocytes. In both models, reversible physiologic changes in myocytes preceded irreversible cell damage. The physiologic changes included reduced amplitude of myocyte contraction, impairment of relaxation and prolongation of the duration of contraction and action potential. In addition, oscillations were noted in the plateau phase of the action potentials. These physiologic changes were accompanied by an early elevation in the cytosolic free calcium concentration, a late elevation in the total exchangeable calcium pool, and attenuation of the [Ca2+]i transient signals. Verapamil inhibited the late elevation in the total exchangeable calcium pool, but failed to inhibit the early elevation in the cytosolic free calcium concentration. These phenomena may explain transient cardiac functional abnormalities that may appear during myocarditis prior to cell destruction. |
| | |
Authors:
|
Y Hasin; Y Eilam; D Hassin; R Fixler |
Related Documents
:
|
19560089 - Mechanisms of stretch-induced atrial fibrillation in the presence and the absence of ad... 8831489 - Role of the kv4.3 k+ channel in ventricular muscle. a molecular correlate for the trans... 10552009 - The antiarrhythmic and anticonvulsant effects of dietary n-3 fatty acids. 1775199 - Effects of the enantiomers of disopyramide and its major metabolite on the electrophysi... 10666079 - Developmental differences in delayed rectifying outward current in feline ventricular m... 11041099 - Preceding stimulus frequency-dependent potentiation of the postrest shortening of the a... 16934799 - Involvement of calcium-mediated apoptotic signals in h2o2-induced min6n8a cell death. 2993039 - Relationships among endogenous digitalis-like factors in essential hypertension. 2419909 - Dihydropyridine-sensitive ca2+ channels in mammalian skeletal muscle cells in culture: ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Advances in experimental medicine and biology Volume: 382 ISSN: 0065-2598 ISO Abbreviation: Adv. Exp. Med. Biol. Publication Date: 1995 |
Date Detail:
|
Created Date: 1996-02-02 Completed Date: 1996-02-02 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0121103 Medline TA: Adv Exp Med Biol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 229-38 Citation Subset: IM |
Affiliation:
|
Cardiology Department, Hadassah Medical Organization, Jerusalem, Israel. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Action Potentials
/
immunology Animals Calcium / physiology* Cell Death Cells, Cultured Coculture Techniques Cytotoxicity, Immunologic Mengovirus / immunology Myocardial Contraction / immunology* Myocardium / cytology, immunology* Rats T-Lymphocytes, Cytotoxic / immunology*, virology |
| Chemical | |
Reg. No./Substance:
|
7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A model approach to the adaptation of cardiac structure by mechanical feedback in the environment of...
Next Document: Na+, K(+)-ATPase and heart excitability.