Document Detail

The effect of cyclic pressure on human monocyte-derived macrophages in vitro.
MedLine Citation:
PMID:  10963180     Owner:  NLM     Status:  MEDLINE    
Aseptic loosening and osteolysis around prosthetic joints are the principal causes of failure and consequent revision. During this process activated macrophages produce cytokines which are thought to promote osteolysis by osteoclasts. Changes in pressure within the space around implants have been proposed as a cause of loosening and osteolysis. We therefore studied the effect of two different regimes of cyclic pressure on the production of interleukin-1beta (IL-1beta), IL-6 and tumour necrosis factor-alpha (TNF-alpha) by cultured human monocyte-derived (M-D) macrophages. There was a wide variation in the expression of cytokines in non-stimulated M-D macrophages from different donors and therefore cells from the same donor were compared under control and pressurised conditions. Both regimes of cyclic pressure were found to increase expression of IL-6 and TNF-alpha. Expression of IL-1beta was increased by a higher-frequency regime only. Our findings suggest that M-D macrophages are activated by cyclic pressure. Further work will be required to understand the relative roles of frequency, amplitude and duration of applied pressure in the cellular effects of cyclic pressure in this system.
G M Ferrier; A McEvoy; C E Evans; J G Andrew
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of bone and joint surgery. British volume     Volume:  82     ISSN:  0301-620X     ISO Abbreviation:  J Bone Joint Surg Br     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2000-09-21     Completed Date:  2000-09-21     Revised Date:  2010-11-10    
Medline Journal Info:
Nlm Unique ID:  0375355     Medline TA:  J Bone Joint Surg Br     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  755-9     Citation Subset:  AIM; IM    
University of Manchester, England.
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MeSH Terms
Cells, Cultured
Interleukin-1 / metabolism*
Interleukin-6 / metabolism*
Macrophages / metabolism*
Osteolysis / metabolism
Tumor Necrosis Factor-alpha / metabolism*
Reg. No./Substance:
0/Interleukin-1; 0/Interleukin-6; 0/Tumor Necrosis Factor-alpha

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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