| The effect of culture media on the production and measurement of luminol-dependent chemiluminescence. | |
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MedLine Citation:
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PMID: 7073957 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of various media constituents on the production and measurement of luminol-dependent chemiluminescence by neutrophils stimulated by opsonized zymosan was studied. Glucose, calcium ions and magnesium ions were found to be necessary for optimal chemiluminescence although high concentrations of glucose had a detrimental effect. For our luminol-dependent system, a pH of 8.5 give maximal readings. The use of simple balanced salt solutions gave higher chemiluminescence responses than did more complex media with added protein, amino acids, and vitamins. However, the latter were important in the maintenance of optimal cell function. |
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Authors:
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M J Hastings; I Petricevic; A J Williams; P J Cole; C S Easmon |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: British journal of experimental pathology Volume: 63 ISSN: 0007-1021 ISO Abbreviation: Br J Exp Pathol Publication Date: 1982 Apr |
Date Detail:
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Created Date: 1982-07-22 Completed Date: 1982-07-22 Revised Date: 2012-05-07 |
Medline Journal Info:
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Nlm Unique ID: 0372543 Medline TA: Br J Exp Pathol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 147-53 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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pharmacology Blood Proteins / pharmacology Calcium / pharmacology Cell Survival Cells, Cultured Culture Media* Glucose / pharmacology Humans Hydrogen-Ion Concentration Luminescent Measurements* Luminol* Magnesium / pharmacology Neutrophils / drug effects Photometry Pyridazines* Vitamins / pharmacology Zymosan |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Blood Proteins; 0/Culture Media; 0/Pyridazines; 0/Vitamins; 50-99-7/Glucose; 521-31-3/Luminol; 7439-95-4/Magnesium; 7440-70-2/Calcium; 9010-72-4/Zymosan |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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