Document Detail


The effect of culture media on the production and measurement of luminol-dependent chemiluminescence.
MedLine Citation:
PMID:  7073957     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of various media constituents on the production and measurement of luminol-dependent chemiluminescence by neutrophils stimulated by opsonized zymosan was studied. Glucose, calcium ions and magnesium ions were found to be necessary for optimal chemiluminescence although high concentrations of glucose had a detrimental effect. For our luminol-dependent system, a pH of 8.5 give maximal readings. The use of simple balanced salt solutions gave higher chemiluminescence responses than did more complex media with added protein, amino acids, and vitamins. However, the latter were important in the maintenance of optimal cell function.
Authors:
M J Hastings; I Petricevic; A J Williams; P J Cole; C S Easmon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of experimental pathology     Volume:  63     ISSN:  0007-1021     ISO Abbreviation:  Br J Exp Pathol     Publication Date:  1982 Apr 
Date Detail:
Created Date:  1982-07-22     Completed Date:  1982-07-22     Revised Date:  2012-05-07    
Medline Journal Info:
Nlm Unique ID:  0372543     Medline TA:  Br J Exp Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  147-53     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / pharmacology
Blood Proteins / pharmacology
Calcium / pharmacology
Cell Survival
Cells, Cultured
Culture Media*
Glucose / pharmacology
Humans
Hydrogen-Ion Concentration
Luminescent Measurements*
Luminol*
Magnesium / pharmacology
Neutrophils / drug effects
Photometry
Pyridazines*
Vitamins / pharmacology
Zymosan
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Blood Proteins; 0/Culture Media; 0/Pyridazines; 0/Vitamins; 50-99-7/Glucose; 521-31-3/Luminol; 7439-95-4/Magnesium; 7440-70-2/Calcium; 9010-72-4/Zymosan
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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