Document Detail


The effect of cryopreservation on parathyroid cell viability and function.
MedLine Citation:
PMID:  9374219     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Higher failure rates have been reported with cryopreserved versus fresh parathyroid autografts. The purpose of this study was to investigate the effects of cryopreservation on live cell yield and to determine if freezing tissue as dispersed cells could improve functional outcome. METHODS: Live cell yield and cytosolic calcium (Ca[i]) and parathyroid hormone (PTH) responses to extracellular calcium were investigated prior to and after 1 to 52 weeks of cryopreservation of bovine parathyroid tissue fragments (group I) or dispersed cells (group II). Tissue was frozen using a liquid nitrogen freezer. RESULTS: A 70% to 90% reduction in live cell yield was observed in groups I and II (P > 0.05), and this was unrelated to duration of cryopreservation. Cytosolic calcium and PTH responses to extracellular calcium in group I were similar to controls. In group II, transient Ca(i) responses were abolished, steady-state Ca(i) responses were blunted (P < 0.05), and PTH secretion was higher than in controls (P < 0.05). CONCLUSIONS: Cryopreservation decreases live cell yield independent of length of storage. Freezing dispersed parathyroid cells produces functional abnormalities in Ca(i) and PTH regulation. Maximizing the amount of tissue for cryopreservation may improve the yield of live cells and the success rate of cryopreserved parathyroid autografts.
Authors:
C R McHenry; D B Stenger; N K Calandro
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of surgery     Volume:  174     ISSN:  0002-9610     ISO Abbreviation:  Am. J. Surg.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1997-12-11     Completed Date:  1997-12-11     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0370473     Medline TA:  Am J Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  481-4     Citation Subset:  AIM; IM    
Affiliation:
Department of Surgery, MetroHealth Medical Center, Case Western Reserve University School of Medicine, Cleveland, Ohio 44109-1998, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / metabolism
Cattle
Cryopreservation*
Graft Survival
Humans
Parathyroid Glands* / cytology,  physiology,  transplantation
Parathyroid Hormone / metabolism
Time Factors
Transplantation, Autologous
Chemical
Reg. No./Substance:
0/Parathyroid Hormone; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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