Document Detail

The effect of cortisol on glycogen and fructose-1,6-bisphosphatase in baby hamster kidney cells infected with Chlamydia trachomatis.
MedLine Citation:
PMID:  6250689     Owner:  NLM     Status:  MEDLINE    
This study was designed to assay changes in glycogen synthesis which may occur as a result of cortisol treatment of chlamydial-infected cells. Monolayers of baby hamster kidney (BHK) cells, unlabeled and prelabeled with [6-14C]glucose, were treated with various concentrations of cortisol before (pretreated) or during (post-treated) infection with Chlamydia trachomatis. At designated times after absorption, the cells were harvested and assayed for total glycogen and 14C accumulation in glycogen. The total amount of glycogen accumulated in cells during the period of greatest chlamydial glycogen synthesis (36 h) was not affected by cortisol treatment. Cortisol treatment appeared to have retarded the accumulation of glycogen in treated infected cells until 30 h after infection. Treated infected cells prelabeled with [6-14C]glucose accumulated a greater amount of 14C in glycogen than untreated infected cells. All cortisol-treated, infected cells exhibited elevated levels of fructose-1,6-bisphosphatase activity, whereas untreated infected cells did not. The hypothesis that cortisol affects chlamydia multiplication by altering the intracellular environment of the host cell is compatible with the results obtained.
S I Reed; W D Hann
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  26     ISSN:  0008-4166     ISO Abbreviation:  Can. J. Microbiol.     Publication Date:  1980 Feb 
Date Detail:
Created Date:  1980-11-24     Completed Date:  1980-11-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  135-45     Citation Subset:  IM    
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MeSH Terms
Cells, Cultured
Chlamydia trachomatis / growth & development*,  metabolism
Fructose-Bisphosphatase / metabolism*
Glycogen / biosynthesis*
Hydrocortisone / pharmacology*
Reg. No./Substance:
50-23-7/Hydrocortisone; 9005-79-2/Glycogen; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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