Document Detail

The effect of corona discharge on Der p 1.
MedLine Citation:
PMID:  11972595     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Reduction of house dust mite allergens in the domestic environment can play an important part in reducing sensitization and in the amelioration of symptoms in atopic individuals. Chemical and physical methods have been tried with varied levels of success. The present paper presents a novel electrostatic way of destroying Der p 1, the major mite allergen. OBJECTIVE: To assess the efficacy of negative Trichel, negative continuous glow, positive pulse and positive continuous glow corona in destroying Der p 1. To determine whether ozone has any effect on the integrity of Der p 1 in the experimental conditions present. METHODS: A simple point-to-plane apparatus was used to irradiate samples of Der p 1 for periods of 1, 15, 30, 45, 60, 120, 180, 240 and 300 min. Controls were exposed to the atmosphere with no corona products present for the equivalent time. The effect of the corona by-product ozone was investigated alone by exposing samples of Der p 1 to molecular ozone for 60 min. Der p 1 concentration was quantified by two-site monoclonal antibody ELISA. RESULTS: High current negative glow resulted in a 67.37% reduction in Der p 1 concentration after 300 min compared with a 50.5% reduction from a low current Trichel regime. High current positive glow corona gave a reduction of 25.22% while a low current positive pulse corona caused a 13.72% reduction after 300 min. All these reductions were statistically significant (P < 0.05) compared with unexposed controls. Negative corona always gave greater percentage reductions in Der p 1 concentration for each time exposure investigated. The pattern of percentage reduction follows an exponential rise to maximum relationship in respect to time. Samples of Der p 1 were not affected by exposure to molecular ozone. CONCLUSION: These data indicate corona products to be a powerful new method of destroying Der p 1 allergen that is not dependent on the presence of the oxidizing corona product ozone.
N Goodman; J F Hughes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology     Volume:  32     ISSN:  0954-7894     ISO Abbreviation:  Clin. Exp. Allergy     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-04-25     Completed Date:  2002-09-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8906443     Medline TA:  Clin Exp Allergy     Country:  England    
Other Details:
Languages:  eng     Pagination:  515-9     Citation Subset:  IM    
Bioelectrostatics Research Centre, Department of Electronics and Computer Science, Faculty of Engineering and Applied Science, University of Southampton, Highfield, Southampton, UK.
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MeSH Terms
Allergens* / analysis
Antigens, Dermatophagoides
Glycoproteins* / analysis
Oxidants, Photochemical / pharmacology
Ozone / pharmacology
Static Electricity
Reg. No./Substance:
0/Allergens; 0/Antigens, Dermatophagoides; 0/Glycoproteins; 0/Ions; 0/Oxidants, Photochemical; 10028-15-6/Ozone
Comment In:
Clin Exp Allergy. 2002 Apr;32(4):483-5   [PMID:  11972590 ]

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