Document Detail

The effect of continuous and interval exercise on PGC-1α and PDK4 mRNA in type I and type II fibers of human skeletal muscle.
MedLine Citation:
PMID:  21883960     Owner:  NLM     Status:  Publisher    
Aim:  Differences in fiber-type recruitment during exercise may induce a heterogenic response in fiber-type gene expression. We have investigated the effect of two different exercise protocols on the fiber type-specific expression of master genes involved in oxidative metabolism (PGC-1α and PDK4). Methods:  Untrained subjects (n=7) completed 90-min cycling either at a constant intensity (CE:∼60% of VO(2max) ) or as interval exercise (IE:∼120/20% VO(2max) , duty cycle 12/18s). Muscle samples were taken before (Pre) and 3 h after (Post) exercise. Single fibers were isolated from freeze-dried muscle and characterized as type I or type II. The cDNA from two fibers of the same type was pooled and mRNA analyzed with reverse transcription quantitative real-time PCR. Results:  CE and IE elicited a small increase in blood lactate (<2.5 mmol l(-1) ) and moderate glycogen depletion (<40%) without difference between exercise mode. The mRNA of PGC-1α and PDK4 increased 5-8 fold in both fiber types after exercise and the relative increase was negatively correlated with the basal level. However, the mRNA of PGC-1α and PDK4 was not different between type I and II fibers neither Pre nor Post and there was no difference in the exercise-induced response between fiber types or exercise modes. Conclusion:  We conclude that the mRNA of PGC-1α and PDK4 increases markedly in both fiber types after prolonged exercise without difference between CE and IE. The similar response between fiber types may relate to that subjects were sedentary and that the metabolic stress was low.
Li Wang; Kent Sahlin
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-25
Journal Detail:
Title:  Acta physiologica (Oxford, England)     Volume:  -     ISSN:  1748-1716     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-9-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101262545     Medline TA:  Acta Physiol (Oxf)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Scandinavian Physiological Society.
GIH, the Swedish School of Sport and Health Sciences, Åstrand Laboratory of work Physiology, Stockholm, Sweden. Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
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