Document Detail


The effect of concanavalin A on egestion of food vacuoles in Tetrahymena.
MedLine Citation:
PMID:  6404645     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability of concanavalin A (conA) to disrupt food vacuole elimination at the cytoproct of Tetrahymena pyriformis, strain GL-C, was investigated using fluorescence microscopy and thin section electron microscopy. ConA was found to induce "tails" in Tetrahymena. These tails were specifically stained by fluorescent conA. Thin section observations of conA-treated cells revealed that these tails were the result of abnormal egestion of food vacuole contents at the cytoproct. Tail formation appears to result from an inhibition of endocytosis of food vacuole membrane during egestion. Instead, the food vacuole membrane appears to be cast out of the cell, along with the contents of the vacuole. The mechanism of this inhibition may be related to an apparent absence of microtubules or microfilamentous mat in the cytoproct region of conA-treated cells. Although conA is ingested into food vacuoles in large amounts, conA appears to affect endocytosis only from outside the cell; ingested conA does not appear to be effective. ConA may exert its influence by binding to the cytoproct region. The ability of conA to induce tail formation is inhibited by sugars specific to it. Numerous membranous vesicles are found in association with the oral cilia and cytoproct region of conA-treated cells. These vesicles may be the conA-binding material reported to be secreted by Tetrahymena.
Authors:
S M Scott; L A Hufnagel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  144     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1983 Apr 
Date Detail:
Created Date:  1983-06-23     Completed Date:  1983-06-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  429-41     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Concanavalin A / pharmacology*
Dose-Response Relationship, Drug
Glucose / pharmacology
Methylmannosides / pharmacology
Microscopy, Electron
Microscopy, Fluorescence
Microtubules / ultrastructure
Organoids / drug effects*
Tetrahymena pyriformis / drug effects,  physiology*,  ultrastructure
Vacuoles / drug effects*
Chemical
Reg. No./Substance:
0/Methylmannosides; 11028-71-0/Concanavalin A; 50-99-7/Glucose; 51023-63-3/methylmannoside

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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