Document Detail


The effect of the cholecystokinin receptor antagonist MK-329 on meal-stimulated pancreaticobiliary output in humans.
MedLine Citation:
PMID:  1568584     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To determine the physiological role of circulating cholecystokinin (CCK), the effect of the CCK receptor antagonist MK-329 on upper digestive processes was investigated in six normal volunteers after a mixed meal. In a double-blind, two-period, randomized crossover design, the subjects received either 10 mg MK-329 or placebo orally 3 hours 15 minutes before the meal, which contained 51CrCl3 as food marker. A five-lumen tube with the tip in the distal duodenum allowed continuous marker infusion (57Co-B12) and duodenal aspiration as well as recordings of antral and duodenal motility patterns via three pressure sensors. Postprandially, MK-329 caused a significant reduction of 30%-60% (P less than 0.05) in pancreatic trypsin output during the initial three 15-minute periods; thereafter, the output was virtually the same than after placebo. Thus, the integrated enzyme response was only reduced by 15% (NS) during the 3-hour period beginning 15 minutes after the meal. In contrast, gallbladder contraction, determined by total bile acid excretion, was inhibited by 77% (P less than 0.05), indicating a crucial role of CCK in regulating gallbladder motility. Except for the initial 30 minutes postprandially, MK-329 also induced a significant reduction in duodenal pH with mean values ranging from 3.5 +/- 0.2 to 4.1 +/- 0.3 compared with 4.5 +/- 0.3 to 5.0 +/- 0.4 after placebo (P less than 0.05), probably because of lowered secretion of pancreatic bicarbonate. Gastric emptying rate was significantly accelerated by MK-329 during the initial 75 minutes after the meal, but the time for 50% emptying did not differ from placebo [127.5 +/- 7.7 vs. 140.0 +/- 9.0 minutes (NS)]. No changes were observed in the motility pattern of the proximal duodenum after feeding. Whereas MK-329 only caused a slight increase of the basal plasma CCK concentrations, the postprandial levels were markedly enhanced. Peak concentrations were 10.0 +/- 1.3 vs. 4.0 +/- 0.5 pmol/L after placebo (P less than 0.001), and the integrated response exceeded the control value by 175% (P less than 0.01). The results suggest that circulating CCK is not an essential mediator of the postprandial pancreatic enzyme secretion in humans, whereas it plays a critical role in gallbladder emptying.
Authors:
P Cantor; P E Mortensen; J Myhre; I Gjorup; H Worning; E Stahl; T T Survill
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Publication Detail:
Type:  Clinical Trial; Journal Article; Randomized Controlled Trial    
Journal Detail:
Title:  Gastroenterology     Volume:  102     ISSN:  0016-5085     ISO Abbreviation:  Gastroenterology     Publication Date:  1992 May 
Date Detail:
Created Date:  1992-05-27     Completed Date:  1992-05-27     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0374630     Medline TA:  Gastroenterology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1742-51     Citation Subset:  AIM; IM    
Affiliation:
Department of Clinical Chemistry and Surgical Gastroenterology C, Rigshospitalet, Copenhagen, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Adult
Benzodiazepinones / pharmacology*
Bile Acids and Salts / secretion
Cholecystokinin / blood
Devazepide
Duodenum / metabolism
Feedback
Food
Gallbladder / drug effects*,  physiology
Gastric Emptying / drug effects
Gastrointestinal Motility
Humans
Hydrogen-Ion Concentration
Male
Pancreas / drug effects*,  secretion
Receptors, Cholecystokinin / antagonists & inhibitors*
Trypsin / secretion
Chemical
Reg. No./Substance:
0/Benzodiazepinones; 0/Bile Acids and Salts; 0/Receptors, Cholecystokinin; 103420-77-5/Devazepide; 9011-97-6/Cholecystokinin; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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