Document Detail


The effect of chemical sympathectomy on mitochondrial function in the ischaemic and reperfused myocardium.
MedLine Citation:
PMID:  7150876     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1 Isolated rabbit hearts were perfused aerobically for 120 min, made ischaemic for 90 min, or made ischaemic for 90 min and then reperfused for 30 min. 2 Some rabbits were pretreated with 6-hydroxydopamine (6-OHDA), given as three separate intravenous doses of 30, 20 and 20 mg/kg, 20 to 48 h before they were killed; others (controls) received saline according to the same regime. 3 Mitochondria were harvested from left ventricular homogenates and their function assessed by measuring state 3O2 consumption (state 3 QO2), respiratory control index (RCI), phosphate: oxygen ratio (ADP:O), Ca2+ content, and ATP-producing activity. In other experiments peak left ventricular developed tension was recorded. 4 In hearts from saline-treated animals, mitochondrial state 3 QO2, RCI and ATP producing activities were reduced after global ischaemia, with or without reperfusion. There was a small gain in mitochondrial Ca2+ after ischaemia, and a large gain upon reperfusion. 5 6-OHDA pretreatment provided some protection against the effects of ischaemia and reperfusion on mitochondrial function and on peak developed tension. 6 It was concluded that chemical sympathectomy with 6-OHDA does not duplicate the effect of prolonged beta-adrenoceptor blockade in protecting mitochondrial function against the deleterious effects of ischaemia and reperfusion.
Authors:
W G Nayler; E M Scott
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  British journal of pharmacology     Volume:  77     ISSN:  0007-1188     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  1982 Dec 
Date Detail:
Created Date:  1983-03-11     Completed Date:  1983-03-11     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  707-15     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Animals
Calcium / metabolism
Catecholamines / metabolism
Coronary Disease / metabolism*
Hydroxydopamines / pharmacology
Male
Mitochondria, Heart / metabolism*
Oxidative Phosphorylation / drug effects
Oxygen Consumption / drug effects
Rabbits
Respiratory Function Tests
Sympathectomy, Chemical*
Chemical
Reg. No./Substance:
0/Catecholamines; 0/Hydroxydopamines; 56-65-5/Adenosine Triphosphate; 7440-70-2/Calcium
Comments/Corrections

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