Document Detail

The effect of cardiac ischemic preconditioning on rat left ventricular gene expression profile.
MedLine Citation:
PMID:  17562528     Owner:  NLM     Status:  MEDLINE    
Ischemic preconditioning (IPC) is a phenomenon where heart is rendered more resistant to subsequent ischemia-reperfusion (I-R)-induced injury by one or more brief episodes of I-R. The mechanisms responsible for cardio-protective effects of IPC are not well characterized. The objective of the study was to characterize gene expression profiles in the left ventricle of male Wistar rat hearts exposed to I-R or IPC followed by I-R. Group 1 included hearts that were only perfused for 30 min. Group 2 included hearts that underwent 30 min perfusion followed by 40 min I and 30 min R. Group 3 comprised 30 min perfused hearts that were subjected to IPC (5 min I + 10 min R + 5 min I + 10 min R) followed by I-R. Total RNAs were isolated from left ventricular tissues. Codelink gene expression system (GE Healthcare) was used for cRNA target preparation, hybridization of microarrays (Rat UniSet 10 K CodeLink bioarrays, GE Healthcare) and detection. Microarrays were scanned with Affymetrix 428 Array scanner. Data analyses were carried out with GeneSifter microarray data analysis software. We determined a total of 140 transcripts (> or =2-fold change) whose expressions were changed (44 up-regulated and 96 down-regulated) accompanying to I-R injury compared to perfused only hearts. Twenty-three transcripts including Ryr3, Crk, Dio1, Npy1r, Ptpra, Cyp51 that were down-regulated by I-R injury, were up-regulated by cardiac IPC. IPC down-regulated the expression of several transcripts including Atf3 (activating transcription factor 3), carboxypeptidase A1 (Cpa1), Slc38a4, Blk which were up-regulated by I-R. In conclusion, evaluation of global gene expression profiling via microarray-based technologies provides a molecular portrait of cardiac IPC of the left ventricular tissue of rat heart.
Halit Canatan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biochemistry and function     Volume:  26     ISSN:  1099-0844     ISO Abbreviation:  Cell Biochem. Funct.     Publication Date:    2008 Mar-Apr
Date Detail:
Created Date:  2008-02-25     Completed Date:  2008-10-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8305874     Medline TA:  Cell Biochem Funct     Country:  England    
Other Details:
Languages:  eng     Pagination:  179-84     Citation Subset:  IM    
Copyright Information:
Copyright 2007 John Wiley & Sons, Ltd.
Department of Pharmacology and Toxicology, Faculty of Medicine, Kuwait University, Safat, Kuwait.
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MeSH Terms
Gene Expression Profiling*
Heart Ventricles / metabolism*
Ischemic Preconditioning, Myocardial*
Myocardium / metabolism*
Oligonucleotide Array Sequence Analysis / methods*
RNA / genetics*
Rats, Wistar
Reg. No./Substance:

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