| The effect of cardiac ischemic preconditioning on rat left ventricular gene expression profile. | |
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MedLine Citation:
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PMID: 17562528 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ischemic preconditioning (IPC) is a phenomenon where heart is rendered more resistant to subsequent ischemia-reperfusion (I-R)-induced injury by one or more brief episodes of I-R. The mechanisms responsible for cardio-protective effects of IPC are not well characterized. The objective of the study was to characterize gene expression profiles in the left ventricle of male Wistar rat hearts exposed to I-R or IPC followed by I-R. Group 1 included hearts that were only perfused for 30 min. Group 2 included hearts that underwent 30 min perfusion followed by 40 min I and 30 min R. Group 3 comprised 30 min perfused hearts that were subjected to IPC (5 min I + 10 min R + 5 min I + 10 min R) followed by I-R. Total RNAs were isolated from left ventricular tissues. Codelink gene expression system (GE Healthcare) was used for cRNA target preparation, hybridization of microarrays (Rat UniSet 10 K CodeLink bioarrays, GE Healthcare) and detection. Microarrays were scanned with Affymetrix 428 Array scanner. Data analyses were carried out with GeneSifter microarray data analysis software. We determined a total of 140 transcripts (> or =2-fold change) whose expressions were changed (44 up-regulated and 96 down-regulated) accompanying to I-R injury compared to perfused only hearts. Twenty-three transcripts including Ryr3, Crk, Dio1, Npy1r, Ptpra, Cyp51 that were down-regulated by I-R injury, were up-regulated by cardiac IPC. IPC down-regulated the expression of several transcripts including Atf3 (activating transcription factor 3), carboxypeptidase A1 (Cpa1), Slc38a4, Blk which were up-regulated by I-R. In conclusion, evaluation of global gene expression profiling via microarray-based technologies provides a molecular portrait of cardiac IPC of the left ventricular tissue of rat heart. |
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Authors:
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Halit Canatan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell biochemistry and function Volume: 26 ISSN: 1099-0844 ISO Abbreviation: Cell Biochem. Funct. Publication Date: 2008 Mar-Apr |
Date Detail:
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Created Date: 2008-02-25 Completed Date: 2008-10-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8305874 Medline TA: Cell Biochem Funct Country: England |
Other Details:
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Languages: eng Pagination: 179-84 Citation Subset: IM |
Copyright Information:
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Copyright 2007 John Wiley & Sons, Ltd. |
Affiliation:
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Department of Pharmacology and Toxicology, Faculty of Medicine, Kuwait University, Safat, Kuwait. canatan@hsc.edu.kw |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Gene Expression Profiling* Heart Ventricles / metabolism* Ischemic Preconditioning, Myocardial* Male Myocardium / metabolism* Oligonucleotide Array Sequence Analysis / methods* RNA / genetics* Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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63231-63-0/RNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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