Document Detail

The effect of carbon tetrachloride and ultraviolet radiation on dolichol levels in liver cells isolated from 3- and 24-month-old male Sprague-Dawley rats.
MedLine Citation:
PMID:  14739707     Owner:  NLM     Status:  MEDLINE    
Dolichol (D) is a long-chain polyprenoid broadly distributed in the cell membranes, possibly endowed with a free-radical scavenging activity, whose concentration in tissues increases with increasing age. No enzyme pathway for D degradation has been discovered. In order to test the hypothesis that D might undergo a non-enzymatic free-radical mediated decomposition the effects of a xenobiotic agent (carbon tetrachloride, CCl(4)) and ultraviolet-B (UV-B) radiation on D levels were studied in liver cells isolated from male ad libitum fed Sprague-Dawley rats aged 3 or 24 months. Liver cells (90 mg/ml) were incubated in sealed flasks (6 ml cell suspension each) for 0, 5, 10 and 20 min after the addition of 25, 50 or 200 microl CCl(4) in the central well. 50 ml of a 6 mg/ml liver cell suspension were poured in a 120 cm(2) Petri dish and the sediment liver cell monolayer was exposed to UVB radiation for 0, 5, 10, 20 and 40 min. At the given time, cells were taken and D was extracted and assayed by the HPLC procedure. D levels were remarkably higher in older than in younger cells as expected ( P < 0.001). Treatment with CCl(4) and UVB caused a highly significant decrease in D ( P < 0.001) whose percentage was larger in younger than in older cells. The conclusions are that free-radicals generated either by chemical or by physical agents cause a very rapid depletion of D in liver cells, and that the effect of the free radical attack on D decomposition may be lower percentage wise in older than in younger cells, which might account at least in part for the accumulation of D in older tissues.
Ilaria Parentini; Ettore Bergamini; Lauretta Cecchi; Gabriella Cavallini; Alessio Donati; Marco Maccheroni; Ilaria Tamburini; Zina Gorii
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biogerontology     Volume:  4     ISSN:  1389-5729     ISO Abbreviation:  Biogerontology     Publication Date:  2003  
Date Detail:
Created Date:  2004-01-23     Completed Date:  2004-08-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100930043     Medline TA:  Biogerontology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  365-70     Citation Subset:  IM    
Centro di Ricerca Interdipartimentale di Biologia e Patologia dell'Invecchiamento, University di Pisa, Pisa, Italy.
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MeSH Terms
Age Factors
Carbon Tetrachloride / metabolism,  pharmacology*
Cells, Cultured
Dolichol / chemistry,  metabolism*
Fatty Acids / chemistry
Free Radicals / metabolism
Hepatocytes / cytology,  drug effects*,  radiation effects*
Random Allocation
Rats, Sprague-Dawley
Ultraviolet Rays*
Reg. No./Substance:
0/Fatty Acids; 0/Free Radicals; 2067-66-5/Dolichol; 56-23-5/Carbon Tetrachloride

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