Document Detail

The effect of calcium ionophore A23187 on the metabolism of arachidonic and eicosapentaenoic acids in neutrophils from a marine teleost fish rich in (n-3) polyunsaturated fatty acids.
MedLine Citation:
PMID:  2822346     Owner:  NLM     Status:  MEDLINE    
1. [1-14C]AA and [1-14C]EPA were incorporated equally into plaice neutrophil phospholipids. 2. Incubation with A23187 resulted in the loss of label from total phospholipids and increased label released from the neutrophils. 3. Labelled LTB4 and LTB5 production was increased 2.5- and 3-fold, respectively, by A23187 treatment. 4. However, the incorporated AA was generally more metabolically active than the incorporated EPA with approx. 3-4 times as much LTB4 produced than LTB5 by cells in the presence or absence of A23187, respectively. 5. The results obtained in this (n-3) PUFA-rich species were discussed in comparison with current knowledge of AA and EPA metabolism in mammalian neutrophils.
D R Tocher; J R Sargent
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Comparative biochemistry and physiology. B, Comparative biochemistry     Volume:  87     ISSN:  0305-0491     ISO Abbreviation:  Comp. Biochem. Physiol., B     Publication Date:  1987  
Date Detail:
Created Date:  1987-12-02     Completed Date:  1987-12-02     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  2984730R     Medline TA:  Comp Biochem Physiol B     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  733-9     Citation Subset:  IM    
Natural Environment Research Council, Institute of Marine Biochemistry, Aberdeen, UK.
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MeSH Terms
Arachidonic Acid
Arachidonic Acids / blood*
Calcimycin / pharmacology*
Carbon Radioisotopes
Eicosapentaenoic Acid / blood*
Flatfishes / blood*
Neutrophils / drug effects,  metabolism*
Phospholipids / biosynthesis,  blood*
Reg. No./Substance:
0/Arachidonic Acids; 0/Carbon Radioisotopes; 0/Phospholipids; 1553-41-9/Eicosapentaenoic Acid; 506-32-1/Arachidonic Acid; 52665-69-7/Calcimycin

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