| The effect of bovine diet on Salmonella survival in synthetic abomasal fluid. | |
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MedLine Citation:
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PMID: 20738440 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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AIMS: To investigate the effect of diet on the survival of Salmonella in the bovine abomasum. METHODS AND RESULTS: Five fistulated cows were randomly assigned to one of five diets denoted as: (i) 100% grass, (ii) grass + 5·3 kg DM concentrate, (iii) 100% grass silage, (iv) 100% hay and (v) maize/grass silage plus concentrates. Rumen fluid was harvested from each dietary treatment and inoculated with nonacid (NA) and acid-adapted (AA) 5-strain Salmonella cocktails. After 24-h incubation period, Salmonella were acid challenged to synthetic abomasum fluid (SAF, pH 2·5) for 5 h to determine their resistance to low pH. The study found that the volatile fatty acids composition and the pH profile of bovine rumen fluid were significantly altered (P <0·05) by some of the dietary treatments but not others. Regression analysis found that significantly higher numbers of acid-adapted Salmonella survived in SAF after incubation in rumen fluid from diets 1, 2 and 4, but fewer significant differences were found between diets for nonacid-adapted Salmonella. The results suggest that the acid-adapted cells were subjected to a higher level of cell injury than the nonadapted cells. CONCLUSIONS: Pre-incubation in rumen fluid did influence the resistance of nonacid and acid-adapted Salmonella to SAF but it was dependant on the dietary treatment fed to the cows. SIGNIFICANCE AND IMPACT OF THE STUDY: This study examined the use of diet, as a modulating factor to limit the bovine excretion of Salmonella with a view to providing a scientific basis for the design of dietary management controls in the future. |
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Authors:
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M Lenahan; S Kelly; S Fanning; D J Bolton |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of applied microbiology Volume: 109 ISSN: 1365-2672 ISO Abbreviation: J. Appl. Microbiol. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9706280 Medline TA: J Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 2060-8 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Journal of Applied Microbiology © 2010 The Society for Applied Microbiology. |
Affiliation:
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Teagasc, Ashtown Food Research Centre, Ashtown, Dublin, Ireland. mary.lenahan@teagasc.ie |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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