Document Detail

The effect of bee pollen on secretion activity, markers of proliferation and apoptosis of porcine ovarian granulosa cells in vitro.
MedLine Citation:
PMID:  21442536     Owner:  NLM     Status:  MEDLINE    
The general objective of this in vitro study was to examine the effect of bee pollen on the release of insulin-like growth factor I (IGF-I) and steroid hormone progesterone, and expression of markers of proliferation (PCNA) and apoptosis (caspase-3) in porcine ovarian granulosa cells. Concentrations of IGF-I and progesterone were determined by RIA method and expression of PCNA and caspase-3 by immunocytochemistry. Bee pollen addition at the dose of 10 ng/mL significantly (P<0.05) inhibited IGF-I release by porcine ovarian granulosa cells. This growth factor was not influenced by 100 and 1000 ng/mL doses of bee pollen. Progesterone release by cells was not influenced by bee pollen addition at the doses of 10, 100 and 1000 ng/mL as used in our study. Similarly expression of PCNA and caspase-3 was not affected by bee pollen addition. The present study shows dose-dependent regulation of IGF-I by experimental bee pollen addition in vitro. Progesterone release, expression of PCNA and caspase-3 in porcine ovarian granulosa cells was not induced by pollen. Our results contribute to new insights regarding the possible effect of bee pollen on IGF-I release, which is important for regulation of porcine ovarian functions.
Adriana Kolesarova; Marcela Capcarova; Zuzana Bakova; Branislav Galik; Miroslav Juracek; Milan Simko; Alexander V Sirotkin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes     Volume:  46     ISSN:  1532-4109     ISO Abbreviation:  J Environ Sci Health B     Publication Date:  2011  
Date Detail:
Created Date:  2011-03-28     Completed Date:  2011-07-18     Revised Date:  2011-09-15    
Medline Journal Info:
Nlm Unique ID:  7607167     Medline TA:  J Environ Sci Health B     Country:  England    
Other Details:
Languages:  eng     Pagination:  207-12     Citation Subset:  IM    
Department of Animal Physiology, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic.
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MeSH Terms
Bees / metabolism*
Caspase 3 / metabolism,  secretion
Cell Proliferation
Cells, Cultured
Granulosa Cells / cytology,  metabolism,  secretion*
Insulin-Like Growth Factor I / metabolism,  secretion
Progesterone / metabolism,  secretion
Reg. No./Substance:
57-83-0/Progesterone; 67763-96-6/Insulin-Like Growth Factor I; EC 3.4.22.-/Caspase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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