Document Detail


The effect of atherosclerosis-induced chronic bladder ischemia on bladder function in the rat.
MedLine Citation:
PMID:  21905085     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
AIMS: To develop a rat model of atherosclerosis-induced chronic bladder ischemia and investigate the effect of chronic bladder ischemia on voiding behavior and bladder function. METHODS: Adult male rats were divided into three groups. The arterial injury (AI) group underwent endothelial injury of the iliac arteries and received a 2% cholesterol diet. The sham group underwent sham operation and received a 2% cholesterol diet. The control group received a regular diet. After 8 weeks, a metabolic cage study and cystometry were performed without anesthesia. Bladder blood flow was measured using a laser Doppler blood flowmeter. Histological examination of the iliac arteries and the bladder was performed. The bladder was also processed for immunohistochemical staining of oxidative stress markers. RESULTS: The metabolic cage study showed that in the AI group, voiding frequency significantly increased while voided volume significantly decreased. Cystometry showed that the frequency of reflex bladder contractions was significantly higher in the AI group. Filling-induced decrease in bladder blood flow was the greatest in the AI group. Histological study showed that in the AI group alone, atherosclerotic occlusion occurred in the iliac arteries as well as in the downstream bladder microvessels. Oxidative stress marker positive cells were more prevalent in the AI bladder than in the other bladders. CONCLUSIONS: Combined with a high-cholesterol diet, endothelial injury of iliac arteries induced arterial occlusive disease in the downstream vessels and consequent bladder ischemia in rats. This model of chronic bladder ischemia showed detrusor overactivity manifested as an increase in voiding frequency. Neurourol. Urodynam. © 2011 Wiley-Liss, Inc.
Authors:
Masanori Nomiya; Osamu Yamaguchi; Karl-Erik Andersson; Koji Sagawa; Ken Aikawa; Keiichi Shishido; Tomohiko Yanagida; Nobuhiro Kushida; Junji Yazaki; Norio Takahashi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-9-8
Journal Detail:
Title:  Neurourology and urodynamics     Volume:  -     ISSN:  1520-6777     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-9-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8303326     Medline TA:  Neurourol Urodyn     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Wiley-Liss, Inc.
Affiliation:
Department of Urology, Fukushima Medical University School of Medicine, Hikarigaoka Fukushima City, Japan; Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Medical Boulevard, Winston-Salem, North Carolina. mnomiya@fmu.ac.jp.
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