Document Detail


Effect of antibiotic treatment on the intestinal metabolome.
MedLine Citation:
PMID:  21282433     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The importance of the mammalian intestinal microbiota to human health has been intensely studied over the past few years. It is now clear that the interactions between human hosts and their associated microbial communities need to be characterized in molecular detail if we are to truly understand human physiology. Additionally, the study of such host-microbe interactions is likely to provide us with new strategies to manipulate these complex systems to maintain or restore homeostasis in order to prevent or cure pathological states. Here, we describe the use of high-throughput metabolomics to shed light on the interactions between the intestinal microbiota and the host. We show that antibiotic treatment disrupts intestinal homeostasis and has a profound impact on the intestinal metabolome, affecting the levels of over 87% of all metabolites detected. Many metabolic pathways that are critical for host physiology were affected, including bile acid, eicosanoid, and steroid hormone synthesis. Dissecting the molecular mechanisms involved in the impact of beneficial microbes on some of these pathways will be instrumental in understanding the interplay between the host and its complex resident microbiota and may aid in the design of new therapeutic strategies that target these interactions.
Authors:
L Caetano M Antunes; Jun Han; Rosana B R Ferreira; Petra Lolić; Christoph H Borchers; B Brett Finlay
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-01-31
Journal Detail:
Title:  Antimicrobial agents and chemotherapy     Volume:  55     ISSN:  1098-6596     ISO Abbreviation:  Antimicrob. Agents Chemother.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-22     Completed Date:  2011-07-15     Revised Date:  2012-05-07    
Medline Journal Info:
Nlm Unique ID:  0315061     Medline TA:  Antimicrob Agents Chemother     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1494-503     Citation Subset:  IM    
Affiliation:
Michael Smith Laboratories, The University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Bacterial Agents / pharmacology*
Eicosanoids / metabolism
Enzyme-Linked Immunosorbent Assay
Female
Intestinal Mucosa / drug effects
Intestines / drug effects*,  metabolism*
Male
Mass Spectrometry
Metabolome / drug effects*
Mice
Mice, Inbred C57BL
Streptomycin / pharmacology
Grant Support
ID/Acronym/Agency:
//Canadian Institutes of Health Research
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Eicosanoids; 57-92-1/Streptomycin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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