| The effect of all-trans-retinoic acid on the synthesis of epidermal cell-surface-associated carbohydrates. | |
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MedLine Citation:
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PMID: 7305988 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. all-trans-Retinoic acid at concentrations greater than 10(-7)m stimulated the incorporation of d-[(3)H]glucosamine into 8m-urea/5% (w/v) sodium dodecyl sulphate extracts of 1m-CaCl(2)-separated epidermis from pig ear skin slices cultured for 18h. The incorporation of (35)SO(4) (2-), l-[(14)C]fucose and U-(14)C-labelled l-amino acids was not significantly affected. 2. Electrophoresis of the solubilized epidermis showed increased incorporation of d-[(3)H]glucosamine into a high-molecular-weight glycosaminoglycan-containing peak when skin slices were cultured in the presence of 10(-5)m-all-trans-retinoic acid. The labelling of other epidermal components with d-[(3)H]glucosamine, (35)SO(4) (2-), l-[(14)C]fucose and U-(14)C-labelled l-amino acids was not significantly affected by 10(-5)m-all-trans-retinoic acid. 3. Trypsinization dispersed the epidermal cells and released 75-85% of the total d-[(3)H]glucosamine-labelled material in the glycosaminoglycan peak. Thus most of this material was extracellular in both control and 10(-5)m-all-trans-retinoic acid-treated epidermis. 4. Increased labelling of extracellular epidermal glycosaminoglycans was also observed when human skin slices were treated with all-trans-retinoic acid, indicating a similar mechanism in both tissues. Increased labelling was also found when the epidermis was cultured in the absence of the dermis, suggesting a direct effect of all-trans-retinoic acid on the epidermis. 5. Increased incorporation of d-[(3)H]-glucosamine into extracellular epidermal glycosaminoglycans in 10(-5)m-all-trans-retinoic acid-treated skin slices was apparent after 4-8h in culture and continued up to 48h. all-trans-Retinoic acid (10(-5)m) did not affect the rate of degradation of this material in cultures ;chased' with 5mm-unlabelled glucosamine after 4 or 18h. 6. Cellulose acetate electrophoresis at pH7.2 revealed that hyaluronic acid was the major labelled glycosaminoglycan (80-90%) in both control and 10(-5)m-all-trans-retinoic acid-treated epidermis. 7. The labelling of epidermal plasma membranes isolated from d-[(3)H]glucosamine-labelled skin slices by sucrose density gradient centrifugation was similar in control and 10(-5)m-all-trans-retinoic acid-treated tissue. 8. The results indicate that increased synthesis of mainly extracellular glycosaminoglycans (largely hyaluronic acid) may be the first response of the epidermis to excess all-trans-retinoic acid. |
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Authors:
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I A King; A Tabiowo |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Biochemical journal Volume: 194 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1981 Jan |
Date Detail:
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Created Date: 1982-01-09 Completed Date: 1982-01-09 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 341-51 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Membrane / drug effects, metabolism Culture Techniques Electrophoresis, Polyacrylamide Gel Epidermis / drug effects, metabolism* Extracellular Space / metabolism Glycoproteins / metabolism Glycosaminoglycans / biosynthesis*, metabolism Swine Tretinoin / pharmacology* Trypsin / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Glycoproteins; 0/Glycosaminoglycans; 302-79-4/Tretinoin; EC 3.4.21.4/Trypsin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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