Document Detail


The effect of aldehyde fixation on selected substrates for energy metabolism and amino acids in mouse brain.
MedLine Citation:
PMID:  78939     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of aldehyde fixation on concentrations of low molecular weight constituents was determined by comparing amounts of selected intermediates in brains of mice exposed to aldehyde fixative solutions with those perfused with phosphate buffer solution alone. Aldehyde perfusion resulted in excellent preservation of cerebral cortex ultrastructure in the presence of dramatic declines in adenosine triphosphate, phosphocreatine, glucose and glucose-6-phosphate that occureed before exposure of the tissue to aldehyde fixatives. Decreases in hexose were accompanied by approximately a 4-fold increase in lactate and a 2-fold increase in pyruvate. Glycogen levels decreased by about 60% during the initial operative procedure but remained constant after aldehyde fixation. Glycogen content declined approximately 90% in tissues that were not treated with aldehyde. Concentrations of aspartate and glutamate changed only slightly during the initial period (1-5 min) and remained constant for at least 90 min in cerebral cortices fixed with aldehydes. Alanine levels increased in both fixed and unfixed tissue; however, this increase was much smaller in tissues exposed promptly to aldehydes. Total ninhydrin-positive material in perchloric acid extracts of brain decreased in mice exposed to aldehyde solutions but increased in tissues that were not. These results indicated that several amino acids may be measured reliably in tissues preserved for light and electron microscopy. In addition, determination of glutamate: alanine ratios in tissues perfused with aldehydes may provide an indication of the timing of fixation.
Authors:
K A Conger; J H Garcia; A S Lossinsky; F C Kauffman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  26     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1978 Jun 
Date Detail:
Created Date:  1978-09-15     Completed Date:  1978-09-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  423-33     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aldehydes*
Amino Acids / metabolism*
Animals
Brain / metabolism*
Cerebral Cortex / metabolism
Energy Metabolism*
Histocytochemistry
Male
Mice
Organophosphorus Compounds / metabolism
Staining and Labeling
Chemical
Reg. No./Substance:
0/Aldehydes; 0/Amino Acids; 0/Organophosphorus Compounds

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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