| The effect of aging on the response of isolated hepatocytes to hydrogen peroxide and tert-butyl hydroperoxide. | |
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MedLine Citation:
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PMID: 19720132 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Aging is associated with increased susceptibility to oxidative stress. To study this in the liver and to elucidate underlying mechanisms, hepatocytes from young (4-6 months) and old (24-26 months) rats were exposed to two oxidants, hydrogen peroxide and tert-butyl hydroperoxide. ATP content and mitochondrial activity were lower in old hepatocytes and decreased further with oxidative stress. Expression of Cu/Zn superoxide dismutase, Mn superoxide dismutase and catalase was not substantially influenced by oxidative stress in young and old hepatocytes, whereas glutathione peroxidase 1 expression was markedly increased only in young hepatocytes. Oxidative stress in young hepatocytes led to increased expression of apoE and movement of apoE to the early endosomes. In old hepatocytes, oxidative stress did not increase apoE expression and apoE was co-localized with early endosomes under control conditions. The results show that old age is associated with impaired hepatocyte responses of mitochondria, ATP, glutathione peroxidase 1 and apoE to oxidative stress. |
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Authors:
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Tharani Sabaretnam; Leonard Kritharides; Jennifer N O'Reilly; David G Le Couteur |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-08-29 |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 24 ISSN: 1879-3177 ISO Abbreviation: Toxicol In Vitro Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-01 Completed Date: 2010-04-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
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Languages: eng Pagination: 123-8 Citation Subset: IM |
Affiliation:
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ANZAC Research Institute, University of Sydney, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Aging / physiology* Animals Antioxidants / metabolism Apolipoproteins E / biosynthesis, metabolism Catalase / metabolism Fluorescent Antibody Technique Gene Expression / drug effects Glutathione Peroxidase / metabolism Hepatocytes / drug effects*, metabolism Hydrogen Peroxide / toxicity* L-Lactate Dehydrogenase / metabolism Male Mitochondria, Liver / drug effects, metabolism Oxidants / toxicity* Oxidative Stress / drug effects Rats Rats, Inbred F344 Reverse Transcriptase Polymerase Chain Reaction Superoxide Dismutase / metabolism tert-Butylhydroperoxide / toxicity* |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Apolipoproteins E; 0/Oxidants; 56-65-5/Adenosine Triphosphate; 75-91-2/tert-Butylhydroperoxide; 7722-84-1/Hydrogen Peroxide; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.11.1.6/Catalase; EC 1.11.1.9/Glutathione Peroxidase; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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