Document Detail


The effect of age of refrigerated conditioned medium on cell survivability in vitro.
MedLine Citation:
PMID:  12656854     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Samples of a conditioned medium (CM), obtained from human ginvigal fibroblasts culture, were kept at 4 degrees C each for 2 weeks, 1, 2, 3 and 6 months. Their effectiveness in maintaining cell survival for their contact with L929 cells in 96 well tissue culture plates for 6 and 10 h at 4 degrees C was compared with Hank's Balanced Salt Solution (HBSS). Surviving cells were stained with neutral red dye and the dye intensity was measured spectrophotometrically at 540 nm. The target cells responded to all the above mentioned CM and the HBSS in the same manner at 6-h contact time. However, by 10-h contact time, the percentage of the target cells' survival was higher in the 2-week, 1- and 2-month CM than in HBSS, 3- and 6-month CM.
Authors:
La-Ongthong Vajrabhaya; Nattaya Vongphan; Pranee Hongskul; Suwanna K Suwannawong
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Dental traumatology : official publication of International Association for Dental Traumatology     Volume:  19     ISSN:  1600-4469     ISO Abbreviation:  Dent Traumatol     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-03-26     Completed Date:  2003-06-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101091305     Medline TA:  Dent Traumatol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  41-4     Citation Subset:  D    
Affiliation:
Department of Operative Dentistry, Faculty of Dentistry, Mahidol University, Yothi Street, Bangkok 10400, Thailand.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Survival
Cells, Cultured
Culture Media, Conditioned*
Fibroblasts
Humans
Isotonic Solutions
L Cells (Cell Line)
Mice
Organ Preservation Solutions*
Refrigeration
Time Factors
Tissue Preservation / methods*
Tooth Avulsion
Tooth Replantation
Chemical
Reg. No./Substance:
0/Culture Media, Conditioned; 0/Hanks Balanced Salt Solution; 0/Isotonic Solutions; 0/Organ Preservation Solutions

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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