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The effect of age on human adipose derived stem cells.
MedLine Citation:
PMID:  22965240     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND:: Adipose derived mesenchymal stem cells (ADSCs) are a robust, multipotent cell source. They exhibit promise in a variety of applications including wound healing, soft-tissue augmentation, and osseous regeneration. They are easily harvested, available among, and utilized across any age-group. The purpose of this study is to evaluate the impact of aging on ADSCs, relating to morphology and senescent properties, as well as, differentiation, growth factor expression, and osteogenesis. We hypothesize that ADSCs from infant donors will exhibit optimized regenerative characteristics compared with older cell sources. METHODS:: Cells were obtained from distinct age-groups (infant, adult, and elderly) and cultured. Morphology was examined using microscopy, and cell surface markers interrogated using flow cytometry. Telomere length, as a function of senescence, was measured using real time PCR. Expression of pertinent angiogenic and osteogenic growth factors were compared. Osteogenic capability was investigated further by evaluating induction response, at different time points, and by quantification of mRNA expression of RUNX-2 and osteocalcin. RESULTS:: The same isolating ratio of mesenchymal stem cells was derived from each donor, regardless of age. The infant ADSCs exhibited elongated spindle morphology, and increased telomere length compared to older cells. Angiogenic factors (VEGF and FGF-2) were more highly expressed by infant ADSCs, whereas osteogenic expression (BMP-2, TGF-B, and SDF-1) were similar among all ages. Response to osteogenic induction was more profound in infant versus older stem cells, as evidenced by ALP and alizarin red staining, as was bone-related gene expression (RUNX-2 and osteocalcin). CONCLUSION:: ADSCs are available across any age-group. Infant-derived ADSCs are morphologically spindle-shaped with long telomeres, and exhibit enhanced angiogenic and osteogenic capabilities, compared to older cell donors. Conversely, all age groups exhibit similar osteogenic paracrine activity and we posit that clinical applicability is conserved during the adult to elderly period.
Authors:
Wei Wu; Laura Niklason; Derek M Steinbacher
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-7
Journal Detail:
Title:  Plastic and reconstructive surgery     Volume:  -     ISSN:  1529-4242     ISO Abbreviation:  Plast. Reconstr. Surg.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1306050     Medline TA:  Plast Reconstr Surg     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
• Yale University School of Medicine - Plastic and Reconstructive Surgery, New Haven, CT * Yale University School of Medicine - Biomedical Engineering, New Haven, CT.
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