| The effect of acrylamide and nitric oxide donors on human mesenchymal progenitor cells. | |
| | |
MedLine Citation:
|
PMID: 22543296 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
We have examined the effects of nitric oxide donors and acrylamide on mesenchymal progenitor cell (hMPC) viability, programmed cell death (PCD) and differentiation. Acrylamide was examined at 0.5mM and 1.5mM concentrations, NOC-18 at 10μM and SNP at 100μM. Cell viability was assayed with MTS, PCD was determined by phosphatidylserine, caspase-9 and -3/7 and mitochondrial membrane potential assays, and osteogenic cell differentiation was evaluated by alkaline phosphatase activity (ALP) and mRNA levels for collagen type I, bone sialoprotein, ostepontin and osteocalcin. Serum-free hMPC cultures treated with 1.5mM acrylamide and SNP for 72h demonstrated reduced viability. PCD analyses revealed that SNP stimulated cells to necrosis in reactive species-dependent manner. Acrylamide led (1.5mM) to apoptosis independent of reactive species. Acrylamide and SNP reduced ALP activity and collagen type I mRNA levels but mRNA levels for bone sialoprotein and osteopontin increased in SNP treated cells and remained unchanged in acrylamide. Acrylamide had no effect on guanylate cyclase and cGMP osteogenic signaling pathway. The study suggests that acrylamide might impair bone development and remodeling upon acute or prolonged intoxication with this compound of mesenchymal cells. |
| | |
Authors:
|
Lukasz Szewczyk; Justyna Ulańska; Marta Dubiel; Anna Maria Osyczka; Grzegorz Tylko |
Related Documents
:
|
22305756 - Myosin-ixa regulates collective epithelial cell migration by targeting rhogap activity ... 15618006 - The hodgkin and reed/sternberg cell. 19417916 - Reusable, compression-sealed fluid cells for surface mounting to planar substrates. 22396456 - Purinergic signalling in the pancreas in health and disease. 21987086 - An immersed boundary method for simulating a single axisymmetric cell growth and division. 23296026 - A new role for micrornas, as ligands of toll-like receptors. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-4-20 |
Journal Detail:
|
Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: - ISSN: 1879-3177 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
|
Created Date: 2012-4-30 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012. Published by Elsevier Ltd. |
Affiliation:
|
Department of Cell Biology and Imaging, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387 Kraków, Poland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Estimating incidence rates with misclassified disease status: a likelihood-based approach, with appl...
Next Document: Kinetic modeling of ?-chloroprene metabolism: Probabilistic in vitro-in vivo extrapolation of metabo...