| The effect of Wag31 phosphorylation on the cells and the cell envelope fraction of wild-type and conditional mutants of Mycobacterium smegmatis studied by visible-wavelength Raman spectroscopy. | |
| | |
MedLine Citation:
|
PMID: 19932688 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Non-surface-enhanced Raman spectroscopy using a 514.5 nm wavelength laser has been used to measure the molecular difference of conditional mutants of Mycobacterium smegmatis expressing three different alleles: wild-type wag31(Mtb), phosphoablative wag31T73A(Mtb), and phosphomimetic wag31T73E(Mtb). This study demonstrates that the phosphorylation of Wag31, a key cell-division protein, causes significant differences in the quantity of amino acids associated with peptidoglycan precursor proteins and lipid II which are observable in the Raman spectra of these cells. Raman spectra were also acquired from the isolated P60 cell envelope fraction of the cells expressing wag31T73A(Mtb) and wag31T73E(Mtb). A significant number of the molecular vibrational differences observed in the cells were also observed in the cell envelope fraction, indicating that these differences are indeed localized in the cell envelope. Principal component analyses and discriminant function analyses were conducted on these data to demonstrate the ease of spectral classification and the reproducibility of the data. |
| | |
Authors:
|
Khozima Hamasha; Moodakare Bheema Sahana; Charul Jani; Seeta Nyayapathy; Choong-Min Kang; Steven J Rehse |
Related Documents
:
|
15847808 - Quantifying cell turnover using cfse data. 17005268 - Sharing the wealth: peroxisome inheritance in budding yeast. 8439588 - The influence of cell co-operation, nutrients and surface area on cell division. 3723058 - The growth of supernumerary legs in the cockroach. 8454338 - Toxicity of pneumolysin to pulmonary alveolar epithelial cells. 14511328 - Expression and activation of caspase 3 following status epilepticus in the rat. |
Publication Detail:
|
Type: Journal Article Date: 2009-11-22 |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: 391 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2010 Jan |
Date Detail:
|
Created Date: 2010-01-27 Completed Date: 2010-03-15 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
|
Languages: eng Pagination: 664-8 Citation Subset: IM |
Copyright Information:
|
Copyright 2009 Elsevier Inc. All rights reserved. |
Affiliation:
|
Department of Physics & Astronomy, Wayne State University, 666 W Hancock, Detroit, MI 48201, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acids Cell Cycle Proteins / chemistry, genetics, metabolism* Cell Membrane / chemistry, metabolism Mutation Mycobacterium smegmatis / genetics, metabolism* Phosphorylation Spectrum Analysis, Raman Uridine Diphosphate N-Acetylmuramic Acid / analogs & derivatives*, chemistry, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 0/Cell Cycle Proteins; 0/Uridine Diphosphate N-Acetylmuramic Acid; 0/muramyl-NAc-(pentapeptide)pyrophosphoryl-undecaprenol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: NSA2, a novel nucleolus protein regulates cell proliferation and cell cycle.
Next Document: Acute myelogenous leukemia cells with the MLL-ELL translocation convert morphologically and function...