Document Detail

The effect of Tuftsin on the nitrous blue tetrazolium reduction of normal human polymorphonuclear leukocytes.
MedLine Citation:
PMID:  1172670     Owner:  NLM     Status:  MEDLINE    
The influence of Tuftsin, the synthetic phagocytosis-stimulating tetrapeptide (L-threonyl-L-lysyl-L-prolyl-L-arginine), on the nitrous blue tetrazolium (NBT) reduction by human polymorphonuclear leukocytes was investigated. It was found that this substance increases the NBT reduction by approximately as much as endotoxin. Other tetrapeptides do not share this property. When Tuftsin analogs are added to the cell suspension and incubated, they prevent the action of both Tuftsin and endotoxin but not of methylene blue. When washed of the analogs, the cells regain the property to be activated by both Tuftsin and endotoxin. It appears that methylene blue on one hand and Tuftsin and endotoxin on the other hand have different sites for their actions. We suggest that whereas methylene blue diffuses into the cell and acts directly upon the hexosemonophosphate shunt activation, Tuftsin and endotoxin appear to act on the cell membrane binding to specific receptors. By treating the cells with Tuftsin analogs, we probably block these receptors.
Z Spirer; V Zakuth; A Golander; N Bogair; M Fridkin
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  55     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  1975 Jan 
Date Detail:
Created Date:  1975-02-18     Completed Date:  1975-02-18     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  198-200     Citation Subset:  AIM; IM    
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MeSH Terms
Arginine / analogs & derivatives,  pharmacology
Cell Nucleus / drug effects
Endotoxins / pharmacology
Immunoglobulin Fragments
Leukocytes / drug effects*
Lipopolysaccharides / pharmacology
Methylene Blue / pharmacology
Oligopeptides / chemical synthesis,  pharmacology*
Spectrum Analysis
Tetrazolium Salts / diagnostic use*
Reg. No./Substance:
0/Endotoxins; 0/Immunoglobulin Fragments; 0/Lipopolysaccharides; 0/Oligopeptides; 0/Tetrazolium Salts; 61-73-4/Methylene Blue; 74-79-3/Arginine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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