Document Detail


The effect of T cell growth factor on the cell cycle of primate T cells.
MedLine Citation:
PMID:  6981667     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of T cell growth factor (TCGF) on the cell cycle of four T cell lines that differed in their response to TCGF was studied. Activated normal marmoset T cells (OH-1) were totally dependent on the addition of TCGF for long-term proliferation. In the absence of TCGF, the cells were incapable of traversing the cell cycle and became arrested in G1. The addition of TCGF to arrested OH-1 cells stimulated them to enter the S phase after a lag phase of 24 to 33 hr. The TCGF-stimulated cells reached a maximum of cells in the S phase by 12 hr after the initiation of DNA synthesis. TCGF was required for a minimum of 18 hr before cells would enter the S phase. In the absence of TCGF, activated owl monkey (8I) and human (RG) lymphocytes displayed a TCGF-sensitive block; addition of TCGF stimulated these cells to enter the S phase after 12 and 16 hr, respectively. In contrast, an owl monkey tumor-derived T cell line (OMT-1), not dependent on exogenous TCGF for proliferation, was able to progress slowly through the cell cycle without a TCGF-sensitive block. These cells responded to TCGF by showing an initial increase in cells that entered the S phase after a lag of 6 hr and a continuing movement of additional cells into S over the course of the next several hours.
Authors:
R L Brown; R L Griffith; R H Neubauer; H Rabin
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  129     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1982 Nov 
Date Detail:
Created Date:  1982-12-03     Completed Date:  1982-12-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1849-53     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Aotus trivirgatus
Cell Cycle
Cell Line
Cell Transformation, Neoplastic / metabolism
Culture Media
DNA / biosynthesis
Humans
Interleukin-2 / pharmacology*
Interphase
Saguinus
T-Lymphocytes / cytology*
Time Factors
Grant Support
ID/Acronym/Agency:
N01-CO-75380/CO/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 0/Interleukin-2; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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