| The effect of Pediococcus acidilactici on the gut microbiota and immune status of on-growing red tilapia (Oreochromis niloticus). | |
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MedLine Citation:
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PMID: 20353430 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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AIM: To assess Pediococcus acidilactici as a dietary supplement for on-growing red tilapia (Oreochromis niloticus). METHODS AND RESULTS: Tilapia were fed either a control diet or control diet supplemented with Ped. acidilactici at 10(7) CFU g(-1) for 32 days. Ped. acidilactici colonized the intestinal tract and significantly affected the intestinal microbial communities. PCR-DGGE revealed direct antagonism of gastric Ped. acidilactici with an endogenous uncultured bacterium during a period of reverting to nonsupplemented feeding. Light microscopy revealed that gut integrity and leucocyte levels were unaffected by Ped. acidilactici; however, blood leucocyte levels and serum lysozyme activity were elevated after 14-days' feeding. No significant improvements in growth performance were observed at the end of the trial (day 32), but survival was significantly higher in the probiotic group. Conclusions: The study demonstrates that oral supplementation of Ped. acidilactici modulates intestinal bacterial communities in on-growing red tilapia and also stimulates some aspects of the nonspecific immune response. Significance and Impact of the study: To our knowledge this is the first study assessing the effects of probiotics on the gut microbiota of tilapia using culture-independent methods. Such methods are crucial to understand the mechanisms which underpin and mediate host benefits. |
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Authors:
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R M W Ferguson; D L Merrifield; G M Harper; M D Rawling; S Mustafa; S Picchietti; J L Balcázar; S J Davies |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of applied microbiology Volume: 109 ISSN: 1365-2672 ISO Abbreviation: J. Appl. Microbiol. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-11-05 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9706280 Medline TA: J Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 851-62 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Journal compilation © 2010 The Society for Applied Microbiology. |
Affiliation:
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Aquaculture and Fish Nutrition Research Group, School of Marine Science and Engineering, Marine Institute, University of Plymouth, Plymouth, UK. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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