Document Detail

The effect of PM10 on human lung fibroblasts.
MedLine Citation:
PMID:  19458133     Owner:  NLM     Status:  MEDLINE    
Asthma diagnoses are increasing nationally with the highest rates in the New England states. Epidemiological studies have suggested a relationship between airborne particulate matter (PM) and severity of an asthma attack. However, because particulate matter, PM, is such a complex mixture, it is difficult to isolate the exacerbating factors. In this paper we investigate the effect of NIST (National Institute of Standards and Technology) and Maine PM and the soluble metals released from the PM on the growth of human lung fibroblasts. While the NIST PM itself had the most pronounced effect on cell survival rates, solutions of metals extracted from the PM also affected cell survival. Treatment of cells with 10, 50, 100 and 200 ug/cm(2) resulted in 84 +/- 13%, 69 +/- 15%, 58 +/- 14% and 58 +/- 16% survival, respectively. Appropriate concentrations of eight acid soluble metals from NIST PM were determined and tested on cells giving 91 +/- 11%, 87 +/- 10%, 72 +/- 18% and 66 +/- 20% survival, respectively. Soluble metals from Maine PM were extracted and mixtures of appropriate concentrations of these metals were used to treat cells, resulting in 88 +/- 5%, 81 +/- 5%, 79 +/- 3% and 57 +/- 9% survival rate. To determine which, if any, of the metals individually affected the cells, Mn, Cu, V and As were used to treat the cells. At the metal concentrations tested, only As and V affected cell survival.
Df Alley; S Langley-Turnbaugh; Nr Gordon; Jp Wise; G Van Epps; A Jalbert
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Toxicology and industrial health     Volume:  25     ISSN:  0748-2337     ISO Abbreviation:  Toxicol Ind Health     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-05-21     Completed Date:  2009-07-07     Revised Date:  2014-08-29    
Medline Journal Info:
Nlm Unique ID:  8602702     Medline TA:  Toxicol Ind Health     Country:  England    
Other Details:
Languages:  eng     Pagination:  111-20     Citation Subset:  IM    
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MeSH Terms
Air Pollutants / analysis,  toxicity*
Arsenic / analysis,  toxicity
Cell Line
Cell Survival / drug effects
Environmental Monitoring
Fibroblasts / drug effects*
Lung / drug effects*
Metals, Heavy / analysis,  toxicity
Particulate Matter / analysis,  toxicity*
Grant Support
Reg. No./Substance:
0/Air Pollutants; 0/Metals, Heavy; 0/Particulate Matter; N712M78A8G/Arsenic

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