Document Detail


The effect of PHA-activated MN-cell supernatants on polymorphonuclear leucocyte function.
MedLine Citation:
PMID:  589867     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of PHA-activated mononuclear-cell (MN) supernatants on various polymorphonuclear-leucocyte (PMN) functions were assessed. Treatment of PMN with PHA-activated MN-cell supernatants resulted in greater electrophoretic mobility, indicating an increase in the negative surface charge. PMN directional motility was inhibited in the presence of active supernatants but was not affected by a pulse exposure of the PMN to these supernatants. Neither control nor active supernatants were chemotactic for PMN, but treatment of these cells with active supernatants produced an increase in their phagocytic activity, their ability to reduce NBT and in their glucose oxidation through the hexosemonophosphate shunt. Bactericidal capacity of these PMN was unaltered. Specific loss of leucocyte inhibitory factor (LIF) activity from supernatants of PHA-activated MN cells followed their absorption with PMN cells but not with human MN cells or guinea-pig peritoneal exudate cells. Furthermore, acquired inhibition of migration of the absorbing PMN was observed.
Authors:
R Lomnitzer; A Glover; A R Rabson
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  29     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  1977 Sep 
Date Detail:
Created Date:  1978-02-18     Completed Date:  1978-02-18     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  501-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Blood Bactericidal Activity
Chemotaxis, Leukocyte
Electrophoresis
Glucose / metabolism
Humans
Leukocytes / immunology
Lymphokines*
Membrane Potentials
Neutrophils / metabolism,  physiology*
Phagocytosis
Chemical
Reg. No./Substance:
0/Lymphokines; 50-99-7/Glucose
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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