Document Detail


The effect of N-acetylcysteine on chloride efflux from airway epithelial cells.
MedLine Citation:
PMID:  19947928     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Defective chloride transport in epithelial cells increases mucus viscosity and leads to recurrent infections with high oxidative stress in patients with CF (cystic fibrosis). NAC (N-acetylcysteine) is a well known mucolytic and antioxidant drug, and an indirect precursor of glutathione. Since GSNO (S-nitrosoglutathione) previously has been shown to be able to promote Cl- efflux from CF airway epithelial cells, it was investigated whether NAC also could stimulate Cl- efflux from CF and non-CF epithelial cells and through which mechanisms. CFBE (CF bronchial epithelial cells) and normal bronchial epithelial cells (16HBE) were treated with 1 mM, 5 mM, 10 mM or 15 mM NAC for 4 h at 37 degrees C. The effect of NAC on Cl- transport was measured by Cl- efflux measurements and by X-ray microanalysis. Cl- efflux from CFBE cells was stimulated by NAC in a dose-dependent manner, with 10 mM NAC causing a significant increase in Cl- efflux with nearly 80% in CFBE cells. The intracellular Cl- concentration in CFBE cells was significantly decreased up to 60% after 4 h treatment with 10 mM NAC. Moreover immunocytochemistry and Western blot experiments revealed expression of CFTR channel on CFBE cells after treatment with 10 mM NAC. The stimulation of Cl- efflux by NAC in CF airway epithelial cells may improve hydration of the mucus and thereby be beneficial for CF patients.
Authors:
Georgia Varelogianni; Igor Oliynyk; Godfried M Roomans; Marie Johannesson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-27
Journal Detail:
Title:  Cell biology international     Volume:  34     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-05     Completed Date:  2010-05-20     Revised Date:  2010-11-12    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  245-52     Citation Subset:  IM    
Affiliation:
Department of Medical Cell Biology, Uppsala University, Box 571, SE-75123 Uppsala, Sweden. georgia.varelogianni@orebroll.se
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MeSH Terms
Descriptor/Qualifier:
Acetylcysteine / pharmacology*
Antioxidants / pharmacology*
Cell Line
Chlorides / metabolism*
Cystic Fibrosis / metabolism
Epithelial Cells / metabolism*
Humans
Immunohistochemistry
Oxidative Stress
Respiratory Mucosa / cytology,  metabolism*
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Chlorides; 616-91-1/Acetylcysteine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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